Shaping the body plan is a complex process that involves the spatial organization and patterning of the different germ layers. Using gastruloids –3D aggregates of mouse embryonic stem cells- we study at cellular resolution the specification of the endoderm. First, we show that contrary to mesoderm, nascent endoderm does not undergo epithelial-mesenchymal transition. Second, we provide evidence for a role of tissue flow, and heterogeneity of cellular junction tension, in the sorting and differentiation of the islets of endoderm cells.


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Hashmi A, Tlili S, Perrin P, Lowndes M, Peradziryi H, Brickman JM, Martínez Arias A, Lenne PF. Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids. Elife. 2022 Apr 11;11:e59371. doi: 10.7554/eLife.59371. PMID: 35404233; PMCID: PMC9033300.


Pierre-François Lenne