Shaping the body plan is a complex process that involves the spatial organization and patterning of the different germ layers. Using gastruloids –3D aggregates of mouse embryonic stem cells- we study at cellular resolution the specification of the endoderm. First, we show that contrary to mesoderm, nascent endoderm does not undergo epithelial-mesenchymal transition. Second, we provide evidence for a role of tissue flow, and heterogeneity of cellular junction tension, in the sorting and differentiation of the islets of endoderm cells.
To know more
Hashmi A, Tlili S, Perrin P, Lowndes M, Peradziryi H, Brickman JM, Martínez Arias A, Lenne PF. Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids. Elife. 2022 Apr 11;11:e59371. doi: 10.7554/eLife.59371. PMID: 35404233; PMCID: PMC9033300.