TEAM

Evolution and development of morphology and behavior

Group leader : B. Prud’homme

Our objective is to elucidate the genetic and cellular mechanisms at the origin of the diversity of shapes and behaviors in animals.

FOR BEGINNERS

Animal species are characterized by a great diversity of morphological forms, color patterns and behaviors. Our research aims to understand how these different traits are encoded by the genetic information contained in the genome, and to elucidate how these traits evolve between species. To do this, we are studying different species of insects, mainly Drosophila flies but also ladybirds. We are studying the formation and evolution of color patterns on the wings of these insects, the morphogenesis of certain body parts, and reproductive behaviors such as courtship or the choice of egg-laying site.

wings

Diversity of wing pigment patterns in Drosophila

We are studying in particular a Drosophila species native to Asia and recently arrived in Europe, Drosophila suzukii. This fly is an agricultural pest that targets many crop fruit while they are still ripening, such as strawberries, cherries, raspberries and many others, causing very important economic losses. Drosophila suzukii behaves unlike most other Drosophila species, which prefer to lay their eggs on decaying fruit. We seek to understand why and how the choice of egg-laying site has changed in Drosophila suzukii. We are studying the genetic mechanisms that control oviposition behavior, but also the formation of the oviposition organ (which has lengthened in Drosophila suzukii compared to other species, allowing it to pierce the skin of ripe fruits). Finally, this species, like some evolutionary close cousins, is recognizable by the presence of a small black spot at the wing tips, but only in males. We are trying to understand how this coloration pattern appeared on the wings of this species, and how it is limited to males, when both sexes share practically the same genome.

 

FOR SPECIALISTS

To study the evolution of shape and behavior in our Drosophila species, we use genetics, genomics, functional transgenesis, imaging, and neural circuit analysis approaches, in a comparative manner between species.

  1. Formation and evolution of coloration patterns.
    The shape of these coloration patterns closely reflects the expression profiles of the genes involved in the appearance of these patterns. Thus, understanding the development and evolution of these pigment patterns essentially means understanding the regulation of the expression of these genes, as well as their evolution. We are therefore seeking to better understand how a particular gene expression profile is encoded by the cis-regulatory sequences of a gene, and how, when applicable, it is sex-specific.
  2. Evolution of epithelial morphogenesis.
    The adult form of animals is dictated by the shape of each of the organs and anatomical parts that compose it. And the shape of the organs is sculpted during embryonic development by the shape and collective organization of the cells that make up that organ. We study the morphogenesis of the egg-laying organ in Drosophila species, and in Drosophila suzukii in particular. To do this, we are studying in a comparative manner how the epithelial cells that form this organ have changed shape and arrangement during evolution. This project is based on genetic, imaging, and image analysis approaches.
  3. Evolution of oviposition site preference.
    Drosophila suzukii behaves inversely to other Drosophila species, preferring to lay eggs on ripe rather than decaying fruit. We seek to identify the signals, especially chemical ones, as well as the neural circuits that perceive and interpret these signals to guide the innate choice towards a favorite oviposition site. We use neural perturbation approaches to characterize and compare neural circuits and the behaviors they govern across species. We are developing genetic tools in Drosophila suzukii to discretely target neuronal populations, and be able to visualize them, record their activity, and manipulate them functionally.

Selected publications

PUBLICATION

Near-chromosome level genome assembly of the fruit pest Drosophila suzukii using long-read sequencing

Mathilde Paris, Roxane Boyer, Rita Jaenichen, Jochen Wolf, Marianthi Karageorgi, Jack Green, Mathilde Cagnon, Hugues Parinello, Arnaud Estoup, Mathieu Gautier, Nicolas Gompel, Benjamin Prud'homme
Sci Rep . 2020 Jul 8;10(1):11227. doi: 10.1038/s41598-020-67373-z. PMID: 32641717

PUBLICATION

Evolution of ovipositor length in Drosophila suzukii is driven by enhanced cell size expansion and anisotropic tissue reorganization

Jack E Green, Matthieu Cavey, Emmanuelle Médina Caturegli, Benoit Aigouy, Nicolas Gompel, Benjamin Prud'homme
Curr Biol . 2019 Jun 17;29(12):2075-2082.e6. doi: 10.1016/j.cub.2019.05.020. Epub 2019 Jun 6. PMID: 31178315

PUBLICATION

A short guide to insect oviposition: when, where and how to lay an egg

Kevin M Cury, Benjamin Prud'homme, Nicolas Gompel
J Neurogenet . Mar-Jun 2019;33(2):75-89. doi: 10.1080/01677063.2019.1586898. PMID: 31164023

PUBLICATION

The genomic basis of color pattern polymorphism in the harlequin ladybird

Mathieu Gautier, Junichi Yamaguchi, Julien Foucaud, Anne Loiseau, Aurélien Ausset, Benoit Facon, Bernhard Gschloessl Jacques Lagnel, Etienne Loire, Hugues Parrinello, Dany Severac, Celine Lopez-Roques, Cecile Donnadieu, Maxime Manno, Helene Berges, Karim Gharbi, Lori Lawson-Handley, Lian-Sheng Zang, Heiko Vogel, Arnaud Estoup, Benjamin Prud'homme
Curr Biol . 2018 Oct 22;28(20):3296-3302.e7. doi: 10.1016/j.cub.2018.08.023. Epub 2018 Aug 23. PMID: 30146156

PUBLICATION

Evolution of Multiple Sensory Systems Drives Novel Egg-Laying Behavior in the Fruit Pest Drosophila suzukii

Karageorgi M, Bräcker LB, Lebreton S, Minervino C, Cavey M, Siju KP, Grunwald Kadow IC, Gompel N, Prud'homme B.
Curr Biol. 2017 Mar 8. pii: S0960-9822(17)30090-8. PMID: 28285999

PUBLICATION

Looking under the lamp post: neither fruiless nor doublesex contribute to the evolution of male courtship in Drosophila

Jessica Cande, David L Stern, Tomoko Morita, Benjamin Prud'homme, Nicolas Gompel
Cell Rep . 2014 Jul 24;8(2):363-70. doi: 10.1016/j.celrep.2014.06.023. Epub 2014 Jul 10. PMID: 25017068

PUBLICATION

Emergence and diversification of a Drosophila pigmentation pattern through the assembly and evolution of a novel gene regulatory module

Laurent Arnoult, Kathy F Y Su, Diogo Manoel, Caroline Minervino, Justine Magriña, Nicolas Gompel, Benjamin Prud'homme
Science . 2013 Mar 22;339(6126):1423-6. doi: 10.1126/science.1233749. PMID: 23520110

PUBLICATION

Body plan innovation in treehoppers through the evolution of an extra wing-like appendage.

Prud'homme B, Minervino C, Hocine M, Cande JD, Aouane A, Dufour HD, Kassner VA, Gompel N.
Nature. 2011 May 5;473(7345):83-6. PMID: 21544145

PUBLICATION

Smells like evolution: the role of chemoreceptor evolution in behavioral change.

Cande J, Prud'homme B, Gompel N.
Curr Opin Neurobiol. 2013 Feb;23(1):152-8. PMID: 22884223

PUBLICATION

Evolution: return of the ant supersoldiers.

Prud'homme B, Gompel N.
Curr Biol. 2012 Mar 6;22(5):R165-7. PMID: 22401899

PUBLICATION

Evolution of multiple additive loci caused divergence between Drosophila yakuba and D. santomea in wing rowing during male courtship.

Cande J, Andolfatto P, Prud'homme B, Stern DL, Gompel N.
PLoS One. 2012;7(8):e43888. PMID: 22952802

PUBLICATION

Behavioural neuroscience: Fruity aphrodisiacs.

Prud'homme B, Gompel N.
Nature. 2011 Oct 12;478(7368):190-1. PMID: 21993752

PUBLICATION

Evolutionary biology: Genomic hourglass.

Prud'homme B, Gompel N.
Nature. 2010 Dec 9;468(7325):768-9. PMID: 21150985

PUBLICATION

Behavioural neurobiology: Chemical love.

Gompel N, Prud'homme B.
Nature. 2009 Oct 15;461(7266):887-8. PMID: 19829359

PUBLICATION

The causes of repeated genetic evolution.

Gompel N, Prud'homme B.
Dev Biol. 2009 Aug 1;332(1):36-47. PMID: 19433086

PUBLICATION

Regulating evolution.

Carroll SB, Prud'homme B, Gompel N.
Sci Am. 2008 May;298(5):60-7. PMID: 18444326

PUBLICATION

Emerging principles of regulatory evolution.

Prud'homme B, Gompel N, Carroll SB.
Proc Natl Acad Sci U S A. 2007 May 15;104 Suppl 1:8605-12. PMID: 17494759

PUBLICATION

Monkey see, monkey do.

Prud'homme B, Carroll SB.
Nat Genet. 2006 Jul;38(7):740-1. PMID: 16804536

PUBLICATION

Repeated morphological evolution through cis-regulatory changes in a pleiotropic gene.

Prud'homme B, Gompel N, Rokas A, Kassner VA, Williams TM, Yeh SD, True JR, Carroll SB.
Nature. 2006 Apr 20;440(7087):1050-3. PMID: 16625197

PUBLICATION

Chance caught on the wing: cis-regulatory evolution and the origin of pigment patterns in Drosophila.

Gompel N, Prud'homme B, Wittkopp PJ, Kassner VA, Carroll SB.
Nature. 2005 Feb 3;433(7025):481-7. PMID: 15690032

Interactions

We collaborate with different teams in France and abroad :

  • chemists at the Institut de Chimie de Nice (ICN),
  • geneticists of population from the Center for Population Biology and Management (CBGP, INRA) in Montpellier,
  • geneticists and neuroscientists from the LMU and TUM in Munich (Germany)

Funding

ANR

Members more

Benoît Aigouy   Matthieu Cavey Bernard Charroux Charalampos-chrysovalantis Galouzis Caroline Minervino Sabine Quitard
Benjamin Prud'homme
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Benjamin Prud'homme

Researcher

Benoît Aigouy
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Benoît Aigouy

Researcher

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Emmanuelle Caturegli

Technical staff

Matthieu Cavey
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Matthieu Cavey

University lecturer

Bernard Charroux
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Bernard Charroux

Researcher

Bernard, CNRS researcher, joined the team in January 2007 coming from the field of developmental biology. Bernard has recently devoted his time in understanding the mechanisms that control the complex relationship that occurs between the microbiota and the gut epithelium. His efforts are also focusing on analyzing the consequences of having a gut-associated microbiota on the physiology of the host.

Charalampos-chrysovalantis Galouzis
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Charalampos-chrysovalantis Galouzis

PhD student

Caroline Minervino
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Caroline Minervino

Technical staff

Sabine Quitard
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Sabine Quitard

Technical staff

Overview

Animal model organism
  • Drosophila species
  • Harmonia axyridis
Biological process studied
  • Gene regulation
  • Comportement
  • Morphogenesis
Biological techniques
  • Genetics
  • Genomics
  • Functional transgenesis
  • Imaging
  • Behavior
  • Neurogenetics