TEAM

Mechanisms of gene regulation by transcription factors

Group leader : Y. Graba ; A. Saurin

We study the function of Hox transcription factors, aimed at identifying the physiological processes under their control as well as the mechanisms underlying specificity and diversity in gene regulation.

FOR BEGINNERS

Gene regulation is central to physiological and pathological processes, and although not exclusively, relies on the activity of transcription factors that form large regulatory multiprotein complexes. Little is known about how such regulatory complexes assemble and what underlies their functional specificity and diversity. Our projects aim at exploring processes controlled by Hox transcription factors, a family of conserved proteins with key roles in development, evolution and diseases, as well as deciphering how they function.

FOR SPECIALISTS

To apprehend how cellular processes are controlled by transcription factors, we focus on the function of the homedomain-containing Hox transcription factors. Hox proteins are encoded by genes usually clustered on the chromosome, with up to 39 genes in vertebrates that can be grouped into 13 paralog groups according to their phylogenetic origin and position within the Hox cluster. Studies have established the importance of Hox proteins in development, with each paralog group protein controlling distinct developmental programs resulting in diversified axial morphogenesis (1). Hox proteins are also central to stem cell and disease biology.

Hox gene complexes and expression in flies and vertebrates

Hox gene complexes and expression in flies and vertebrates

Despite our extensive knowledge into the developmental function of Hox proteins, the extent of cellular processes controlled by Hox and how Hox proteins achieve transcriptional regulation requires much more insight. Our research activity falls into two main directions:

The first research direction aims at characterizing novel cellular processes controlled by Hox proteins. While well-studied for their role in promoting axial differential morphogenesis, where distinct Hox paralogs provide distinct functions, we recently uncovered cellular processes where Hox proteins seems to act in a non-paralog specific manner (2,3). This is illustrated by the regulation of basal cellular processes such as autophagy.

Generic and specific Hox functions

Generic and specific Hox functions

The second research direction aims at understanding how Hox proteins function at the mechanistic level, including situations where they act in a paralog non-specific manner. Most of our knowledge on Hox protein mode of action so far stems from studying their interaction with PBC class cofactors, particularly within their paralog specific axial positioning function. We aim at identifying novel Hox cofactors, in particular those sheding light on the transcriptional mechanisms underlying their non-paralog specific functions.

Generic and specific Hox functions

M1BP, an RNA PolII pausing factor and novel Hox cofactor

The approach taken is multidisciplinary in nature, using genomics, transcriptomics and proteomics to set the regulatory landscape; biochemistry, structural biology and molecular modelling to grasp the atomic details of the mechanisms, and molecular genetics to probe in vivo function. Integration of this novel knowledge should draw novel aspects of the biology and mechanisms of Hox protein functions. We believe this is central to understand the bases for gene regulation during development and evolution, and in the longer term for manipulating transcription factor activity and gene regulation in stem cell and disease biology.

(1) – Rezsohazy R., Saurin A. J., Maurel-Zaffran C. and Graba Y. (2015). Cellular and molecular insights into Hox protein action. Development, Apr 1;142(7):1212-1227. Review
(2) – Banreti A, Hudry B, Sass M, Saurin AJ, Graba Y( 2014). Hox proteins mediate developmental and environmental control of autophagy. Developmental Cell. Jan 13;28(1):56-69.
(3) – Saurin AJ, Delfini MC, Maurel-Zaffran C, Graba Y (2018) The Generic Facet of Hox Protein Function. Trends in Genetics. Sep 18. pii: S0168-9525(18)30138-0.
(4) – Zouaz A, Auradkar A, Delfini MC, Macchi M, Barthez M, Ela Akoa S, Bastianelli L, Xie G, Deng WM, Levine SS, Graba Y, Saurin AJ (2017). The Hox proteins Ubx and AbdA collaborate with the transcription pausing factor M1BP to regulategene transcription. EMBO J. Oct 2;36(19):2887-2906.

Selected publications

PUBLICATION

Stem cell regionalization during olfactory bulb neurogenesis depends on regulatory interactions between Vax1 and Pax6

Nathalie Coré, Andrea Erni, Hanne M Hoffmann, Pamela L Mellon, Andrew J Saurin, Christophe Beclin, Harold Cremer
Elife . 2020 Aug 7;9:e58215. doi: 10.7554/eLife.58215. PMID: 32762844

PUBLICATION

Human ZKSCAN3 and Drosophila M1BP are functionally homologous transcription factors in autophagy regulation

Marine Barthez, Mathilde Poplineau, Marwa Elrefaey, Nathalie Caruso, Yacine Graba, Andrew J Saurin
Sci Rep . 2020 Jun 15;10(1):9653. doi: 10.1038/s41598-020-66377-z PMID: 32541927

PUBLICATION

Loss of bhlha9 impairs thermotaxis and formalin-evoked pain in a sexually dimorphic manner

Manon Bohic, Irène Marics, Catarina Santos, Pascale Malapert, Nissim Ben Arie, Chiara Salio, Ana Reynders, Yves Le Feuvre, Andrew J.Saurin, Aziz Moqrich
Cell Rep, 30 (3), 602-610.e6 2020 Jan 21 PMID: 31968239

PUBLICATION

Cooperation of axial and sex specific information controls Drosophila female genitalia growth by regulating the Decapentaplegic pathway

Jesús Romero-Pozuelo, David Foronda, Paloma Martín, Bruno Hudry, Samir Merabet, Yacine Graba, Ernesto Sánchez-Herrero
Dev Biol . 2019 Oct 15;454(2):145-155. doi: 10.1016/j.ydbio.2019.06.014. Epub 2019 Jun 25. PMID: 31251896

PUBLICATION

PLZF limits enhancer activity during hematopoietic progenitor aging

Mathilde Poplineau, Julien Vernerey, Nadine Platet, Lia N'guyen, Léonard Hérault, Michela Esposito, Andrew J Saurin, Christel Guilouf, Atsushi Iwama, Estelle Duprez
Nucleic Acids Res . 2019 May 21;47(9):4509-4520. doi: 10.1093/nar/gkz174 PMID: 30892634

PUBLICATION

The Generic Facet of Hox Protein Function

Andrew J Saurin, Marie Claire Delfini, Corinne Maurel-Zaffran, Yacine Graba
Trends Genet . 2018 Dec;34(12):941-953. doi: 10.1016/j.tig.2018.08.006 PMID: 30241969

PUBLICATION

Post-translational modifications of HOX proteins, an underestimated issue

Amandine Draime, Laure Bridoux, Yacine Graba, René Rezsohazy
Int J Dev Biol . 2018;62(11-12):733-744. doi: 10.1387/ijdb.180178rr PMID: 30604843

PUBLICATION

Fattening the perspective of Hox protein specificity through SLiMming

Lucrezia Rinaldi, Andrew J Saurin, Yacine Graba
Int J Dev Biol . 2018;62(11-12):755-766. doi: 10.1387/ijdb.180306yg PMID: 30604845

PUBLICATION

Hypermethylation of gene body CpG islands predicts high dosage of functional oncogenes in liver cancer

Arechederra M, Daian F, Yim A, Bazai SK, Richelme S, Dono R, Saurin AJ, Habermann BH, Maina F.
Nat Commun. 2018 Aug 8;9(1):3164. doi: 10.1038/s41467-018-05550-5. PMID: 30089774

PUBLICATION

The Hox proteins Ubx and AbdA collaborate with the transcription pausing factor M1BP to regulate gene transcription.

Zouaz A, Auradkar A, Delfini MC, Macchi M, Barthez M, Ela Akoa S, Bastianelli L, Xie G, Deng WM, Levine SS, Graba Y, Saurin AJ.
EMBO J. 2017 Oct 2;36(19):2887-2906. doi: 10.15252/embj.201695751. PMID: 28871058

PUBLICATION

Hox functional diversity: Novel insights from flexible motif folding and plastic protein interaction

Ortiz-Lombardia M, Foos N, Maurel-Zaffran C, Saurin AJ, Graba Y.
Bioessays. 2017 Apr;39(4). doi: 10.1002/bies.201600246. PMID: 28092121

PUBLICATION

Hox Service Warranty Extends to Adult Bone Repair.

Saurin AJ, Delfini MC, Graba Y
Dev Cell. 2016 Dec 19;39(6):627-629. doi: 10.1016/j.devcel.2016.12.007. PMID: 27997818

PUBLICATION

Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis

Kerridge S, Munjal A, Philippe JM, Jha A, de las Bayonas AG, Saurin AJ, Lecuit T.
Nat Cell Biol. 2016 Mar;18(3):261-70. doi: 10.1038/ncb3302. PMID: 26780298

PUBLICATION

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).

Klionsky et al.
Autophagy. 2016;12(1):1-222. doi: 10.1080/15548627.2015.1100356. PMID: 26799652

PUBLICATION

A survey of conservation of sea spider and Drosophila Hox protein activities.

Saadaoui M, Litim-Mecheri I, Macchi M, Graba Y, Maurel-Zaffran C.
Mech Dev. 2015 Nov;138 Pt 2:73-86. PMID: 26238019

PUBLICATION

Correlation between low FAT1 expression and early affected muscle in facioscapulohumeral muscular dystrophy

Mariot V, Roche S, Hourdé C, Portilho D, Sacconi S, Puppo F, Duguez S, Rameau P, Caruso N, Delezoide AL, Desnuelle C, Bessières B, Collardeau S, Feasson L, Maisonobe T, Magdinier F, Helmbacher F, Butler-Browne G, Mouly V, Dumonceaux J.
Ann Neurol. 2015 Sep;78(3):387-400. doi: 10.1002/ana.24446. PMID: 26018399

PUBLICATION

Cellular and molecular insights into Hox protein action.

Rezsohazy R, Saurin AJ, Maurel-Zaffran C, Graba Y.
Development. 2015 Apr 1;142(7):1212-27. PMID: 25804734

PUBLICATION

A flexible extension of the Drosophila ultrabithorax homeodomain defines a novel Hox/PBC interaction mode.

Foos N, Maurel-Zaffran C, Maté MJ, Vincentelli R, Hainaut M, Berenger H, Pradel J, Saurin AJ, Ortiz-Lombardía M, Graba Y.
Structure. 2015 Feb 3;23(2):270-9. doi: 10.1016/j.str.2014.12.011. PMID: 25651060

PUBLICATION

Autophagy : Moving Benchside Promises to Patient Bedsides.

Belaid A, Ndiaye PD, Filippakis H, Roux J, Rottinger E, Graba Y, Brest P, Hofman P, Mograbi B.
Curr Cancer Drug Targets. 2015;15(8):684-702. PMID: 26452384

PUBLICATION

Drosophila Hox transcription factors access the RNA Polymerase II machinery through direct homeodomain binding to a conserved motif of Mediator subunit Med19

Boube M, Hudry B, Immarigeon C, Carrier Y, Bernat-Fabre S, Merabet S, Graba Y, Bourbon HM, Cribbs DL.
PLoS Genet. 2014 May 1;10(5):e1004303. PMID: 24786462

PUBLICATION

Hox genes : a fertile interplay of concepts and methods.

Graba Y. and Rezsohazy R.
Methods Mol Biol., 1196, Editors. doi: 10.1007/978-1-4939-1242-1-18.

PUBLICATION

Hox proteins mediate developmental and environmental control of autophagy.

Banreti A, Hudry B, Sass M, Saurin AJ, Graba Y.
Dev Cell. 2014 Jan 13;28(1):56-69. PMID: 24389064

PUBLICATION

Distinct genetic requirements for BX-C mediated specification of abdominal denticles.

Sambrani N, Pradel J, Graba Y.
Dev Dyn. 2013 Oct 24. doi: 10.1002/dvdy.24081. PMID: 24155218

PUBLICATION

The emerging role of acetylation in the regulation of autophagy.

Bánréti A, Sass M, Graba Y.
Autophagy. 2013 Jun 1;9(6):819-29. PMID: 23466676

PUBLICATION

Distinct molecular strategies for Hox-mediated limb suppression in Drosophila: from cooperativity to dispensability/antagonism in TALE partnership.

Sambrani N, Hudry B, Zouaz A, Maurel-Zaffran C, Merabet S and Graba Y.
PLoS Genet. 2013;9(3):e1003307. PMID: 23505377

PUBLICATION

Antagonism Versus Cooperativity with TALE Cofactors at the Base of the Functional Diversification of Hox Protein Function.

Rivas ML, Espinosa-Vázquez JM, Sambrani N, Greig S, Merabet S,Graba Y, Castelli-Gair Hombría J.
PLoS Genet. 2013 Feb;9(2):e1003252. PMID: 23408901

PUBLICATION

Hox proteins display a common and ancestral ability to diversify their interaction mode with the PBC class cofactors.

Hudry B, Remacle S, Delfini MC, Rezsohazy R, Graba Y, Merabet S.
PLoS Biol. 2012;10(6):e1001351. PMID: 22745600

PUBLICATION

The MYST-containing protein Chameau is required for proper sensory organ specification during Drosophila thorax morphogenesis.

Hainaut M, Sagnier T, Berenger H, Pradel J, Graba Y, Miotto B.
PLoS One. 2012;7(3):e32882. PMID: 22412942

PUBLICATION

Insights into Hox protein function from a large scale combinatorial analysis of protein domains.

Merabet S, Litim-Mecheri I, Karlsson D, Dixit R, Saadaoui M, Monier B, Brun C, Thor S, Vijayraghavan K, Perrin L, Pradel J, Graba Y.
PLoS Genet. 2011 Oct;7(10):e1002302. PMID: 22046139

PUBLICATION

Characterisation of genome-wide PLZF/RARA target genes.

Spicuglia S, Vincent-Fabert C, Benoukraf T, Tibéri G, Saurin AJ, Zacarias-Cabeza J, Grimwade D, Mills K, Calmels B, Bertucci F, Sieweke M, Ferrier P,Duprez E.
PLoS One. 2011;6(9):e24176. PMID: 21949697

PUBLICATION

Selection of distinct Hox-Extradenticle interaction modes fine-tunes Hox protein activity.

Saadaoui M, Merabet S, Litim-Mecheri I, Arbeille E, Sambrani N, Damen W, Brena C, Pradel J, Graba Y.
Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2276-81. PMID: 21262810

PUBLICATION

Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay.

Hudry B, Viala S, Graba Y, Merabet S.
BMC Biol. 2011 Jan 28;9:5. PMID: 21276241

PUBLICATION

Control of DNA replication: a new facet of Hox proteins?

Miotto B, Graba Y.
Bioessays. 2010 Sep;32(9):800-7. PMID: 20730947

PUBLICATION

Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.

Maurel-Zaffran C, Pradel J, Graba Y.
Dev Biol. 2010 Jul 1;343(1-2):18-27. PMID: 20403348

PUBLICATION

Regulation of Hox activity: insights from protein motifs.

Merabet S, Sambrani N, Pradel J, Graba Y.
Adv Exp Med Biol. 2010;689:3-16. PMID: 20795319

PUBLICATION

The PRC1 Polycomb group complex interacts with PLZF/RARA to mediate leukemic transformation

Boukarabila H, Saurin AJ, Batsché E, Mossadegh N, van Lohuizen M, Otte AP, Pradel J, Muchardt C, Sieweke M, Duprez E.
Genes Dev. 2009 May 15;23(10):1195-206. doi: 10.1101/gad.512009. PMID: 19451220

PUBLICATION

Classification of sequence signatures: a guide to Hox protein function.

Merabet S, Hudry B, Saadaoui M, Graba Y.
Bioessays. 2009 May;31(5):500-11. PMID: 19334006

PUBLICATION

Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.

Diop SB, Bertaux K, Vasanthi D, Sarkeshik A, Goirand B, Aragnol D, Tolwinski NS, Cole MD, Pradel J, Yates JR 3rd, Mishra RK, Graba Y, Saurin AJ.
EMBO Rep. 2008 Mar;9(3):260-6. PMID: 18259215

PUBLICATION

A unique Extradenticle recruitment mode in the Drosophila Hox protein Ultrabithorax.

Merabet S, Saadaoui M, Sambrani N, Hudry B, Pradel J, Affolter M,Graba Y.
Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16946-51. PMID: 17942685

PUBLICATION

Chameau HAT and DRpd3 HDAC function as antagonistic cofactors of JNK/AP-1-dependent transcription during Drosophila metamorphosis.

Miotto B, Sagnier T, Berenger H, Bohmann D, Pradel J, Graba Y.
Genes Dev. 2006 Jan 1;20(1):101-12. PMID: 16391236

PUBLICATION

Getting a molecular grasp on Hox contextual activity.

Merabet S, Pradel J, Graba Y.
Trends Genet. 2005 Sep;21(9):477-80. PMID: 16023254

PUBLICATION

Hox-controlled reorganisation of intrasegmental patterning cues underlies Drosophila posterior spiracle organogenesis.

Merabet S, Hombria JC, Hu N, Pradel J, Graba Y.
Development. 2005 Jul;132(13):3093-102. PMID: 15930099

PUBLICATION

Tgfbeta signaling acts on a Hox response element to confer specificity and diversity to Hox protein function.

Grienenberger A, Merabet S, Manak J, Iltis I, Fabre A, Bérenger H, Scott MP, Pradel J, Graba Y.
Development. 2003 Nov;130(22):5445-55. PMID: 14507783

PUBLICATION

The hexapeptide and linker regions of the AbdA Hox protein regulate its activating and repressive functions.

Merabet S, Kambris Z, Capovilla M, Bérenger H, Pradel J, Graba Y.
Dev Cell. 2003 May;4(5):761-8. PMID: 12737810

PUBLICATION

Hox genes and apoptosis, from architecture to sculpture.

Pradel J, Graba Y, Aragnol D.
Med Sci (Paris). 2003 Mar;19(3):271-3. PMID: 12836405

PUBLICATION

A green fluorescent protein reporter genetic screen that identifies modifiers of Hox gene function in the Drosophila embryo.

Merabet S, Catala F, Pradel J, Graba Y.
Genetics. 2002 Sep;162(1):189-202. PMID: 12242233

PUBLICATION

The MYST domain acetyltransferase Chameau functions in epigenetic mechanisms of transcriptional repression.

Grienenberger A, Miotto B, Sagnier T, Cavalli G, Schramke V, Geli V, Mariol MC, Berenger H, Graba Y, Pradel J.
Curr Biol. 2002 Apr 30;12(9):762-6. PMID: 12007422

PUBLICATION

DWnt4 and wingless elicit similar cellular responses during imaginal development.

Gieseler K, Wilder E, Mariol MC, Buratovitch M, Bérenger H, Graba Y, Pradel J.
Dev Biol. 2001 Apr 15;232(2):339-50. PMID: 11401396

PUBLICATION

Dynamic expression of d-CdGAPr, a novel Drosophila melanogaster gene encoding a GTPase activating protein.

Sagnier T, Grienenberger A, Mariol M, Bérenger H, Pradel J, Graba Y.
Mech Dev. 2000 Jun;94(1-2):267-70. PMID: 10842085

PUBLICATION

Distinct hox protein sequences determine specificity in different tissues.

Chauvet S, Merabet S, Bilder D, Scott MP, Pradel J, Graba Y.
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4064-9. PMID: 10737765

PUBLICATION

Plasticity versus specificity in RTK signalling modalities for distinct biological outcomes in motor neurons

Caruso N, Herberth B, Lamballe F, Arce-Gorvel V, Maina F, Helmbacher F.
BMC Biol. 2014 Aug 14;12(1):56. PMID: 25124859

PUBLICATION

TAFA4, a Chemokine-like Protein, Modulates Injury-Induced Mechanical and Chemical Pain Hypersensitivity in Mice.

Delfini MC, Mantilleri A, Gaillard S, Hao J, Reynders A, Malapert P, Alonso S, François A, Barrere C, Seal R, Landry M, Eschallier A, Alloui A, Bourinet E, Delmas P, Le Feuvre Y, Moqrich A.
Cell Rep. 2013 Oct 31;5(2):378-88. PMID: 24139797

PUBLICATION

Deregulation of the Protocadherin Gene FAT1 Alters Muscle Shapes: Implications for the Pathogenesis of Facioscapulohumeral Dystrophy.

Caruso N, Herberth B, Bartoli M, Puppo F, Dumonceaux J, Zimmermann A, Denadai S, Lebossé M, Roche S, Geng L, Magdinier F, Attarian S, Bernard R, Maina F, Levy N, Helmbacher F.
PLoS Genet. 2013 Jun;9(6):e1003550. PMID: 23785297

PUBLICATION

Pool-specific regulation of motor neuron survival by neurotrophic support.

Lamballe F, Genestine M, Caruso N, Arce V, Richelme S, Helmbacher F*, Maina F*. (* co-senior authors)
J Neurosci. 2011 Aug 3;31(31):11144-58. PMID: 21813676

PUBLICATION

Stable, conditional, and muscle-fiber-specific expression of electroporated transgenes in chick limb muscle cells

Wang H, Bonnet A, Delfini MC, Kawakami K, Takahashi Y, Duprez D.
Dev Dyn. 2011 May;240(5):1223-32. doi: 10.1002/dvdy.22498. PMID: 21509896

PUBLICATION

The timing of emergence of muscle progenitors is controlled by an FGF/ERK/SNAIL1 pathway

Delfini MC, De La Celle M, Gros J, Serralbo O, Marics I, Seux M, Scaal M, Marcelle C.
Dev Biol. 2009 Sep 15;333(2):229-37. doi: 10.1016/j.ydbio.2009.05.544. PMID: 19445915

PUBLICATION

Telomeric trans-silencing in Drosophila melanogaster: tissue specificity, development and functional interactions between non-homologous telomeres

Josse T, Maurel-Zaffran C, de Vanssay A, Teysset L, Todeschini AL, Delmarre V, Chaminade N, Anxolabéhère D, Ronsseray S.
PLoS One. 2008 Sep 22;3(9):e3249. doi: 10.1371/journal.pone.0003249. PMID: 18813361

PUBLICATION

A molecular clock operates during chick autopod proximal-distal outgrowth

Pascoal S, Carvalho CR, Rodriguez-León J, Delfini MC, Duprez D, Thorsteinsdóttir S, Palmeirim I.
J Mol Biol. 2007 Apr 27;368(2):303-9. PMID: 17346744

PUBLICATION

Liprin-alpha has LAR-independent functions in R7 photoreceptor axon targeting.

Hofmeyer K, Maurel-Zaffran C, Sink H, Treisman JE.
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11595-600. PMID: 16864797

PUBLICATION

Control of the segmentation process by graded MAPK/ERK activation in the chick embryo

Delfini MC, Dubrulle J, Malapert P, Chal J, Pourquié O.
Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11343-8. PMID: 16055560

PUBLICATION

Ectopic Myf5 or MyoD prevents the neuronal differentiation program in addition to inducing skeletal muscle differentiation, in the chick neural tube

Delfini MC, Duprez D.
Development. 2004 Feb;131(4):713-23. PMID: 14724123

PUBLICATION

Reptin and pontin antagonistically regulate heart growth in zebrafish embryos.

Rottbauer W, Saurin AJ, Lickert H, Shen X, Burns CG, Wo ZG, Kemler R, Kingston R, Wu C, Fishman M.
Cell. 2002 Nov 27;111(5):661-72. PMID: 12464178

PUBLICATION

Direct imaging of human SWI/SNF-remodeled mono- and polynucleosomes by atomic force microscopy employing carbon nanotube tips

Schnitzler GR, Cheung CL, Hafner JH, Saurin AJ, Kingston RE, Lieber CM.
Mol Cell Biol. 2001 Dec;21(24):8504-11. http://www.pubmed.com/11713285/

PUBLICATION

Cell-autonomous and -nonautonomous functions of LAR in R7 photoreceptor axon targeting.

Maurel-Zaffran C, Suzuki T, Gahmon G, Treisman JE, Dickson BJ.
Neuron. 2001 Oct 25;32(2):225-35. PMID: 11683993

PUBLICATION

Reconstitution of a functional core polycomb repressive complex.

Francis NJ, Saurin AJ, Shao Z, Kingston RE.
Mol Cell. 2001 Sep;8(3):545-56. http://www.pubmed.com/11583617/

PUBLICATION

A Drosophila Polycomb group complex includes Zeste and dTAFII proteins

Saurin AJ, Shao Z, Erdjument-Bromage H, Tempst P, Kingston RE.
Nature. 2001 Aug 9;412(6847):655-60. PMID: 11493925

PUBLICATION

Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes.

Sif S, Saurin AJ, Imbalzano AN, Kingston RE.
Genes Dev. 2001 Mar 1;15(5):603-18. PMID: 11238380

PUBLICATION

Notch signalling acts in postmitotic avian myogenic cells to control MyoD activation

Hirsinger E, Malapert P, Dubrulle J, Delfini MC, Duprez D, Henrique D, Ish-Horowicz D, Pourquié O.
Development. 2001 Jan;128(1):107-16. PMID: 11092816

PUBLICATION

Delta 1-activated notch inhibits muscle differentiation without affecting Myf5 and Pax3 expression in chick limb myogenesis

Delfini MC, Hirsinger E, Pourquié O, Duprez D.
Development. 2000 Dec;127(23):5213-24. PMID: 11060246

PUBLICATION

HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties

Bárdos JI, Saurin AJ, Tissot C, Duprez E, Freemont PS.
J Biol Chem. 2000 Sep 15;275(37):28785-92. PMID: 10825164

PUBLICATION

Paraxis is expressed in myoblasts during their migration and proliferation in the chick limb bud

Mech Dev. 2000 Sep;96(2):247-51. PMID: 10960793

PUBLICATION

dlarp, a new candidate Hox target in Drosophila whose orthologue in mouse is expressed at sites of epithelium/mesenchymal interactions

Chauvet S, Maurel-Zaffran C, Miassod R, Jullien N, Pradel J, Aragnol D.
Dev Dyn. 2000 Jul;218(3):401-13. PMID: 10878606

PUBLICATION

The Ste20 kinase misshapen regulates both photoreceptor axon targeting and dorsal closure, acting downstream of distinct signals

Su YC, Maurel-Zaffran C, Treisman JE, Skolnik EY.
Mol Cell Biol. 2000 Jul;20(13):4736-44. PMID: 10848599

PUBLICATION

pannier acts upstream of wingless to direct dorsal eye disc development in Drosophila

Maurel-Zaffran C, Treisman JE.
Development. 2000 Mar;127(5):1007-16. PMID: 10662640

PUBLICATION

nessy, an evolutionary conserved gene controlled by Hox proteins during Drosophila embryogenesis

Maurel-Zaffran C, Chauvet S, Jullien N, Miassod R, Pradel J, Aragnol D.
Mech Dev. 1999 Aug;86(1-2):159-63. PMID: 10446276

PUBLICATION

Antagonist activity of DWnt-4 and wingless in the Drosophila embryonic ventral ectoderm and in heterologous Xenopus assays.

Gieseler K, Graba Y, Mariol MC, Wilder EL, Martinez-Arias A, Lemaire P, Pradel J.
Mech Dev. 1999 Jul;85(1-2):123-31. PMID: 10415353

PUBLICATION

SSX and the synovial-sarcoma-specific chimaeric protein SYT-SSX co-localize with the human Polycomb group complex.

Soulez M, Saurin AJ, Freemont PS, Knight JC.
Oncogene. 1999 Apr 29;18(17):2739-46. PMID: 10348348

PUBLICATION

Identification of the t(15;17) in AML FAB types other than M3: evaluation of the role of molecular screening for the PML/RARalpha rearrangement in newly diagnosed AML. The Medical Research Council (MRC) Adult Leukaemia Working Party

Allford S, Grimwade D, Langabeer S, Duprez E, Saurin A, Chatters S, Walker H, Roberts P, Rogers J, Bain B, Patterson K, McKernan A, Freemont P, Solomon E, Burnett A, Goldstone A, Linch D.
Br J Haematol. 1999 Apr;105(1):198-207. PMID: 10233384

PUBLICATION

SUMO-1 modification of the acute promyelocytic leukaemia protein PML: implications for nuclear localisation

Duprez E, Saurin AJ, Desterro JM, Lallemand-Breitenbach V, Howe K, Boddy MN, Solomon E, de Thé H, Hay RT, Freemont PS.
J Cell Sci. 1999 Feb;112 ( Pt 3):381-93. http://www.pubmed.com/9885291/

PUBLICATION

The human polycomb group complex associates with pericentromeric heterochromatin to form a novel nuclear domain

Saurin AJ, Shiels C, Williamson J, Satijn DP, Otte AP, Sheer D, Freemont PS.
J Cell Biol. 1998 Aug 24;142(4):887-98. PMID: 9722603

PUBLICATION

Wnt and TGFbeta signals subdivide the AbdA Hox domain during Drosophila mesoderm patterning.

Bilder D, Graba Y, Scott MP.
Development. 1998 May;125(9):1781-90. PMID: 9521915

PUBLICATION

RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.

Satijn DP, Gunster MJ, van der Vlag J, Hamer KM, Schul W, Alkema MJ, Saurin AJ, Freemont PS, van Driel R, Otte AP.
Mol Cell Biol. 1997 Jul;17(7):4105-13. PMID: 9199346

PUBLICATION

Drosophila Hox complex downstream targets and the function of homeotic genes.

Graba Y, Aragnol D, Pradel J.
Bioessays. 1997 May;19(5):379-88. PMID: 9174403

PUBLICATION

Hox genes in evolution: protein surfaces and paralog groups.

Sharkey M, Graba Y, Scott MP.
Trends Genet. 1997 Apr;13(4):145-51. PMID: 9097725

PUBLICATION

The Drosophila teashirt homeotic protein is a DNA-binding protein and modulo, a HOM-C regulated modifier of variegation, is a likely candidate for being a direct target gene.

Alexandre E, Graba Y, Fasano L, Gallet A, Perrin L, De Zulueta P, Pradel J, Kerridge S, Jacq B.
Mech Dev. 1996 Oct;59(2):191-204. PMID: 8951796

PUBLICATION

Does this have a familiar RING?

Saurin AJ, Borden KL, Boddy MN, Freemont PS.
Trends Biochem Sci. 1996 Jun;21(6):208-14. PMID: 8744354

PUBLICATION

Inhibition of mitogen-induced DNA synthesis by bafilomycin A1 in Swiss 3T3 fibroblasts

Saurin AJ, Hamlett J, Clague MJ, Pennington SR.
Biochem J. 1996 Jan 1;313 ( Pt 1):65-70. PMID: 8546711

PUBLICATION

wingless and DWnt4, 2 Drosophila Wnt genes, have related expression, regulation and function during the embryonic development.

Gieseler K, Mariol MC, Sagnier T, Graba Y, Pradel J.
C R Acad Sci III. 1995 Nov;318(11):1101-10. PMID: 8574787

PUBLICATION

Genetic and molecular analysis of terminal deletions of chromosome 3R of Drosophila melanogaster.

Laurenti P, Graba Y, Rosset R, Pradel J.
Gene. 1995 Mar 10;154(2):177-81. PMID: 7890161

PUBLICATION

DWnt-4, a novel Drosophila Wnt gene acts downstream of homeotic complex genes in the visceral mesoderm.

Graba Y, Gieseler K, Aragnol D, Laurenti P, Mariol MC, Berenger H, Sagnier T, Pradel J.
Development. 1995 Jan;121(1):209-18. PMID: 7867502

PUBLICATION

The modifier of variegation modulo gene acts downstream of dorsoventral and HOM-C genes and is required for morphogenesis in Drosophila.

Graba Y, Laurenti P, Perrin L, Aragnol D, Pradel J.
Dev Biol. 1994 Dec;166(2):704-15. PMID: 7813788

PUBLICATION

A quick method for immunoscreening recombinant bacterial colonies.

Laurenti P, Graba Y, Pradel J.
Trends Genet. 1993 Oct;9(10):335-6. PMID: 8273145

PUBLICATION

Cell lineage-specific expression of modulo, a dose-dependent modifier of variegation in Drosophila.

Garzino V, Pereira A, Laurenti P, Graba Y, Levis RW, Le Parco Y, Pradel J.
EMBO J. 1992 Dec;11(12):4471-9. PMID: 1425581

PUBLICATION

Homeotic control in Drosophila; the scabrous gene is an in vivo target of Ultrabithorax proteins.

Graba Y, Aragnol D, Laurenti P, Garzino V, Charmot D, Berenger H, Pradel J.
EMBO J. 1992 Sep;11(9):3375-84. PMID: 1505521

Interactions

IBDM
Other National and international :

Funding

ARC funding for Y. Graba Team from IBDM, IBDML

FRM funding for Y.Graba Team from IBDM, IBDML

ligue contre le canceramidex

Members more

Nathalie Caruso Marie-claire Delfini Farcot Corinne Maurel Zaffran Gabriela Poliacikova Axelle Wilmerding
Andrew Saurin
Close window
Andrew Saurin

Researcher

Andrew Saurin obtained his PhD from King’s College, University of London, and did his postdoc at Harvard University. Andrew joined the team in 2002 on a CNRS permanent research position. Background and main expertise: Gene regulation through epigenetic mechanisms, protein and chromatin complex biochemistry.

Yacine Graba
Close window
Yacine Graba

Researcher

Nathalie Caruso
Close window
Nathalie Caruso

Technical staff

Marie-claire Delfini Farcot
Close window
Marie-claire Delfini Farcot

Researcher

Corinne Maurel Zaffran
Close window
Corinne Maurel Zaffran

University lecturer

Corinne Maurel-Zaffran obtained her PhD in 1997 from the University of Aix-Marseille II. Following two post docs at New York University Medical center and University of Paris 6, Corinne joined the team in September 2003 as assistant Professor and is teaching genetics and molecular biology at the bachelor and master levels. Background and main expertise: developmental gene regulation, drosophila molecular genetics and imaging.

Gabriela Poliacikova
Close window
Gabriela Poliacikova

PhD student

Axelle Wilmerding
Close window
Axelle Wilmerding

PhD student

Alumni

  • A. Zouaz, PhD
  • S. Merabet, CR CNRS
  • M. Hainaut, PhD
  • I. Litim, PhD 
  • M. Saadaoui, PhD 
  • N. Sambrani, PhD
  • B. Hudry, PhD
  • B. Miotto, PhD
  • A. Grienenberger, PhD
  • R. Rodriguez, Post doc
  • K. Bertaux, Post doc
  • S. Viala, CDD

Overview

Animal model organism
Biological process studied
  • Gene regulation, transcription
Biological techniques
  • Molecular genetics
  • Genomics
  • Proteomics
  • Biochemistry
  • Bioinformatics
  • Structural biology
  • Molecular modeling