Agenda
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Monday, 15 November 2021 13:30
External SeminarLaurent Blanchoin
CEA, Grenoble
- A. Michelot
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Thursday, 18 November 2021 12:30
Internal SeminarRandom Illumination Microscopy for Biology
Simon Labouesse
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Thursday, 25 November 2021 12:30
Internal SeminarStriatal cholinergic interneurons and stereotyped behaviors in Tshz3 deletion-linked autism spectrum disorder
Jordan Molitor
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Friday, 26 November 2021 11:30
External SeminarThe axonal cytoskeleton at the nanoscale
Christophe Leterrier
Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, Marseille, France
- H. Cremer
- Hybrid seminar: 40 person in Amphi12 (with registration), the other on Zoom
AbstractThe intricate morphology and molecular identity of axons is maintained for decades, but also continuously adapts to changes in the environment and activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and mobility of axonal components. In our lab, we use super-resolution microscopy to map the nanoscale architecture actin-based structures within the axon. In the axon initial segment, a key compartment for the maintenance of neuronal polarity, we resolved a highly organized assembly encompassing the periodic actin/spectrin scaffold and its partners: ankyrin, myosin. We have also visualized new actin structures along the axon shaft: rings, hotspots and trails, and are now exploring their molecular organization and functions as well as the role of actin at presynapses. For this, we develop a combination of versatile labeling, correlative live-cell/super-resolution/electron microscopy and quantitative, deep-learning based analysis that allow for high-content, nanoscale interrogation of the axonal architecture.