© Elodie Lanet – Maurange Team

Engineers and technicians from the IBDM imaging platform, identified as «Regroupement inter-organismes (Rio)» in 2003, and IBiSA («Infrastructures Biologie Santé et Agronomie») since 2008 under the Picsl body ensure the availability of modern and innovative biophotonic technologies for researchers from the institute and for the local and national scientific community. Their activities include the acquisition and management of a range of microscopes, user and PhD training (microscopes, image processing and analysis) and integration of new imaging systems (non-linear imaging, high resolution imaging) through technology transfer from research teams (P.F. Lenne team) thanks to BioImaging France (FBI), a national infrastructure investments for the future. The imaging resource platform adheres to strict quality controls (ISO 9001 standard). Equipment performance and stability are ensured through metrological monitoring.

Message to users : you should indicate in your publications (acknowledgments paragraph) the following formula with the ANR Convention number : We acknowledge France–‐BioImaging infrastructure supported by the French National Research Agency (ANR–‐10–‐INBS–‐04–‐01, «Investments for the future»).

The platform is a FBI’s member.

• High resolution microscopy
• Full field microscopy 
• Confocal microscopy
• Two-photon microscopy
• Objectifs
• Metrology

HIGH RESOLUTION MICROSCOPY

STED Time-gated room
XY resolution reached 50 nm
Inverted microscope with white laser and 592 nm continuous laser depletion
2 hybrids detectors, 100 x and 60 x magnification

FULL FIELD MICROSCOPY

AxioImager M2 Apotome (room 1023)
Upright microscope with epifluorescence filter: Dapi, CFP, GFP, Rhod, Texas Red, Cy5
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack

AxioImager Z1 Apotome (room 1023)
Upright microscope with epifluorescence filter: Dapi, CFP, GFP, YFP, Rhod, Texas Red, Cy5
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack, MosaiX

AxioObserver Z1 Apotome (salle 1023)
Inverted microscope with epifluorescence filter: Dapi, GFP, Rhod
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack, Time Laps, Mark&Find

Axioplan2Imaging Apotome (room 1023)
Upright microscope with epifluorescence filter: Dapi, GFP, Rhod, Texas Red, Cy5
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack, Time Laps

AxioObserver Z1 (room 7014)
Inverted microscope with epifluorescence filter: Rhod and diodes 365nm, 470nm, 590nm
Temperature and CO2 controlled room
Imagery: Multichannel, Zstack, Time Laps, Mark&Find

DMRXA2 (room 928)
Upright microscope with epifluorescence filter: Dapi, GFP, Rhod
Full fields speed imaging with Cascade camera 128 * 128 pixel driven by Métamorph

CONFOCAL MICROSCOPY

LSM 510 META (room 928)
upright microscope Zeiss Axioskop
Excitation lines available: 405, 458, 477, 488, 514, 543 et 633 nm
Controlled by LSM software

LSM 510 (room 926)
Inverted microscope Zeiss Axiovert200M
Excitation lines available: 458, 477, 488, 514, 543 et 633 nm
Controlled by ZEN 2008 software

LSM 510 META (room 926)
Inverted microscope Zeiss Axiovert200M
Excitation lines available: 405, 458, 477, 488, 514, 543 et 633 nm
Controlled by LSM software

LSM 780 (room 928)
Upright microscope Zeiss AxioImager Z2
Excitation lines available: 405, 458, 488, 514, 561 et 633 nm
Equipped with a highly sensitive GaAsP detector for a range of specific wavelength
FCS (Fluorescence correlation spectrometry) Module
Controlled by ZEN 2010 software

Spinning disk Perkin’s Elmer 1 caméra (room 928)
Upright microscope Zeiss Axiovert 200M
Excitation lines available: 488 et 561 nm
Equipped with a photometric camera coolsnap HQ and controlled by Metamorph

Spinning disk Roper 2 caméras (room 924)
Inverted microscope Nikon Eclilpse TI
Excitation lines available: 405, 445, 491, 515, 561 et 633 nm
Equipped with 2 cameras Photometrics EMCCD evolve
Controlled by Metamorph, dedicated for fast imaging

Biorad MRC 1024 ES (room 924)
Microscope Nikon Diaphot 300
Confocal microscope specializing in UV
Educational equipment

TWO-PHOTON MICROSCOPY

Zeiss 7M, ZEN software
Upright microscope with environmental control
5 non descanned detectors
Laser Mai tai deepsee HP

Inverted microscope with environnemental control
2 non descanned  detectors
Laser Mai tai deepsee HP

OBJECTIFS

AxioImager Z1 Apo (room 1023): 5x/0.5 Plan Néofluar; 10x/0.3 Plan Néofluar; 20x/0.8 Plan Apochromat; 40x/1.3 Plan Néofluar; 63x/1.4 Plan Apochromat.

AxioImager M2 Apo (room 1023): 5x/0.5 Plan Néofluar; 10x/0.3 Plan Néofluar; 20x/0.75 Plan Apochromat; 40x/1.3 Plan Néofluar; 63x/1.4 Plan Apochromat.

Axioplan 2 (room 1023): 5x/0.5 Plan Néofluar; 10x/0.3 Plan Néofluar Ph1; 25x/0.80 Imm Korr Néofluar; 40x/1.3 Plan Néofluar Ph2; 63x/1.4 Plan Apochromat DicIII.

AxioObserver Z1 Apo (room 1023): 2.5x/0.075 Plan Néofluar; 5x/0.16 Plan; 10x/0.3 Plan Néofluar Ph1; 20x/0.4 LD Plan Néofluar Ph2; 40x/0.60 LD Plan Néofluar Ph2 DicII; 63x/1.4 oil Plan Apochromat.

Imager M2 (room 730): 5x/0.15 Plan Néofluar; 10x/0.30 Plan Néofluar Ph1 DicI; 20x/0.5 Plan Néofluar DicII; 16x/0.5 Imm Plan Néofluar Ph2; 40x/1.30 oil Plan Néofluar Ph3 DicIII; 63x/1.40 oil Plan Apochromat Ph3 DicIII.

DMRB (room 730): 5x/0.11 NPlan; 10x/0.40 Imm PL APO Dic; 20x/0.5 Fluotar DIC; 40x/0.7 Fluotar Dic.

Axioplan2 (room 7014): 10x/0.30 W Ph1 Achroplan; 5x/0.15 Plan Néofluar; 10x/0.30 Plan Néofluar DicI; 20x/0.5 Plan Néofluar DicII; 40x/0.75 Plan Néofluar; 63x/1.25 oil Plan Néofluar DicIII.

AxioObserver Z1 (room 7014): 2.5x/0.075 Plan; 10x/0.3 Plan Néofluar Ph1; 20x/0.40 LD Plan Néofluar Ph2 DicII; 40x/0.6 LD Plan Néofluar Ph2 DicII; 63x/1.4 oil Plan Apochromat.

LSM 510 (room 926):10x/0.3 Plan Neofluar; 20x/0.8 Plan Apochromat; 25x/0.8 LCI Plan Neofluar; 40x/1.2 C Apochromat; 63x/1.4 Plan Apochromat; 100X/1.3 Plan Neofluar.

LSM 510 Meta (room 926):10x/0.3 Plan Neofluar; 20x/0.75 Plan Apochromat; 40x/1.3 Plan Neofluar; 40x/1.2 C Apochromat; 63x/1.4 Plan Apochromat; 100X/1.3 Plan Neofluar.

LSM 780 (room 928):10x/0.45 Plan Apochromat; 20x/0.8 Plan Apochromat; 25x/0.8 LD LCI Plan Apochromat; 40x/1.2 C Apochromat; 40x/1.4 Plan Apochromat; 63x/1.4 Plan Apochromat.

Spinning Roper 2 cameras (room 924):10x/0.3 Plan Fluor; 20X/0.45 S Plan Fluor ELWD; 20x/0.75 Plan Fluor; 40x/0.6 S Plan Fluor ELWD; 100X/1.4 Plan Apo VC.

METROLOGY

• Monitoring devices
• Instruments / methodology