Platform

Optical imaging

Scientific manager : P.F. Lenne
Technical manager : C. Matthews

© Elodie Lanet – Maurange Team

Engineers and technicians from the IBDM imaging platform, identified as «Regroupement inter-organismes (Rio)» in 2003, and IBiSA («Infrastructures Biologie Santé et Agronomie») since 2008 under the Picsl body ensure the availability of modern and innovative biophotonic technologies for researchers from the institute and for the local and national scientific community. Their activities include the acquisition and management of a range of microscopes, user and PhD training (microscopes, image processing and analysis) and integration of new imaging systems (non-linear imaging, high resolution imaging) through technology transfer from research teams (P.F. Lenne team) thanks to BioImaging France (FBI), a national infrastructure investments for the future. The imaging resource platform adheres to strict quality controls (ISO 9001 standard). explant155 tshz3Equipment performance and stability are ensured through metrological monitoring.

Message to users : you should indicate in your publications (acknowledgments paragraph) the following formula with the ANR Convention number : We acknowledge France–‐BioImaging infrastructure supported by the French National Research Agency (ANR–‐10–‐INBS–‐04–‐01, «Investments for the future»).

The platform is a FBI’s member.

PUBLICATION

MetroloJ : an ImageJ plugin to help monitor microscopes' health Cédric Matthews 1,3 and Fabrice P. Cordelières 2,3

PUBLICATION

Bull Cancer. 2007 Jan;94(1):115-25. Theory, principles and applications of fluorescent technologies in cellular biology and cancer research.

PUBLICATION

Three-dimensional imaging of small intestine morphology using non-linear optical microscopy and endogenous signals.

Ricard C, Vacca B, Weber P.
J Anat. 2012 Sep;221(3):279-83. PMID: 22697278
TEXTE

HIGH RESOLUTION MICROSCOPY

stedSTED Time-gated room
XY resolution reached 50 nm
Inverted microscope with white laser and 592 nm continuous laser depletion
2 hybrids detectors, 100 x and 60 x magnification

 

 

 

TEXTE

FULL FIELD MICROSCOPY

axioimagerAxioImager M2 Apotome (room 1023)
Upright microscope with epifluorescence filter: Dapi, CFP, GFP, Rhod, Texas Red, Cy5
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack

 

display88AxioImager Z1 Apotome (room 1023)
Upright microscope with epifluorescence filter: Dapi, CFP, GFP, YFP, Rhod, Texas Red, Cy5
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack, MosaiX

 

display92AxioObserver Z1 Apotome (salle 1023)
Inverted microscope with epifluorescence filter: Dapi, GFP, Rhod
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack, Time Laps, Mark&Find

display89Axioplan2Imaging Apotome (room 1023)
Upright microscope with epifluorescence filter: Dapi, GFP, Rhod, Texas Red, Cy5
Structured illumination module (Apotome) controlled by Axiovision
Imagery: Multichannel, Zstack, Time Laps

display98
AxioObserver Z1 (room 7014)
Inverted microscope with epifluorescence filter: Rhod and diodes 365nm, 470nm, 590nm
Temperature and CO2 controlled room
Imagery: Multichannel, Zstack, Time Laps, Mark&Find

display94DMRXA2 (room 928)
Upright microscope with epifluorescence filter: Dapi, GFP, Rhod
Full fields speed imaging with Cascade camera 128 * 128 pixel driven by Métamorph

TEXTE

 

CONFOCAL MICROSCOPY

display99LSM 510 META (room 928)
upright microscope Zeiss Axioskop
Excitation lines available: 405, 458, 477, 488, 514, 543 et 633 nm
Controlled by LSM software

 

 

display86LSM 510 (room 926)
Inverted microscope Zeiss Axiovert200M
Excitation lines available: 458, 477, 488, 514, 543 et 633 nm
Controlled by ZEN 2008 software

 

display82LSM 510 META (room 926)
Inverted microscope Zeiss Axiovert200M
Excitation lines available: 405, 458, 477, 488, 514, 543 et 633 nm
Controlled by LSM software

 

display100

LSM 780 (room 928)
Upright microscope Zeiss AxioImager Z2
Excitation lines available: 405, 458, 488, 514, 561 et 633 nm
Equipped with a highly sensitive GaAsP detector for a range of specific wavelength
FCS (Fluorescence correlation spectrometry) Module
Controlled by ZEN 2010 software

 

 

display96Spinning disk Perkin’s Elmer 1 caméra (room 928)
Upright microscope Zeiss Axiovert 200M
Excitation lines available: 488 et 561 nm
Equipped with a photometric camera coolsnap HQ and controlled by Metamorph

 

display97Spinning disk Roper 2 caméras (room 924)
Inverted microscope Nikon Eclilpse TI
Excitation lines available: 405, 445, 491, 515, 561 et 633 nm
Equipped with 2 cameras Photometrics EMCCD evolve
Controlled by Metamorph, dedicated for fast imaging

display99Biorad MRC 1024 ES (room 924)
Microscope Nikon Diaphot 300
Confocal microscope specializing in UV
Educational equipment

 

 

 

 

TEXTE

 

TWO-PHOTON MICROSCOPY

display84Zeiss 7M, ZEN software
Upright microscope with environmental control
5 non descanned detectors
Laser Mai tai deepsee HP

 

 

display84Inverted microscope with environnemental control
2 non descanned  detectors
Laser Mai tai deepsee HP

 

 

TEXTE

 

OBJECTIFS

AxioImager Z1 Apo (room 1023): 5x/0.5 Plan Néofluar; 10x/0.3 Plan Néofluar; 20x/0.8 Plan Apochromat; 40x/1.3 Plan Néofluar; 63x/1.4 Plan Apochromat.

AxioImager M2 Apo (room 1023): 5x/0.5 Plan Néofluar; 10x/0.3 Plan Néofluar; 20x/0.75 Plan Apochromat; 40x/1.3 Plan Néofluar; 63x/1.4 Plan Apochromat.

Axioplan 2 (room 1023): 5x/0.5 Plan Néofluar; 10x/0.3 Plan Néofluar Ph1; 25x/0.80 Imm Korr Néofluar; 40x/1.3 Plan Néofluar Ph2; 63x/1.4 Plan Apochromat DicIII.

AxioObserver Z1 Apo (room 1023): 2.5x/0.075 Plan Néofluar; 5x/0.16 Plan; 10x/0.3 Plan Néofluar Ph1; 20x/0.4 LD Plan Néofluar Ph2; 40x/0.60 LD Plan Néofluar Ph2 DicII; 63x/1.4 oil Plan Apochromat.

Imager M2 (room 730): 5x/0.15 Plan Néofluar; 10x/0.30 Plan Néofluar Ph1 DicI; 20x/0.5 Plan Néofluar DicII; 16x/0.5 Imm Plan Néofluar Ph2; 40x/1.30 oil Plan Néofluar Ph3 DicIII; 63x/1.40 oil Plan Apochromat Ph3 DicIII.

DMRB (room 730): 5x/0.11 NPlan; 10x/0.40 Imm PL APO Dic; 20x/0.5 Fluotar DIC; 40x/0.7 Fluotar Dic.

Axioplan2 (room 7014): 10x/0.30 W Ph1 Achroplan; 5x/0.15 Plan Néofluar; 10x/0.30 Plan Néofluar DicI; 20x/0.5 Plan Néofluar DicII; 40x/0.75 Plan Néofluar; 63x/1.25 oil Plan Néofluar DicIII.

AxioObserver Z1 (room 7014): 2.5x/0.075 Plan; 10x/0.3 Plan Néofluar Ph1; 20x/0.40 LD Plan Néofluar Ph2 DicII; 40x/0.6 LD Plan Néofluar Ph2 DicII; 63x/1.4 oil Plan Apochromat.

LSM 510 (room 926):10x/0.3 Plan Neofluar; 20x/0.8 Plan Apochromat; 25x/0.8 LCI Plan Neofluar; 40x/1.2 C Apochromat; 63x/1.4 Plan Apochromat; 100X/1.3 Plan Neofluar.

LSM 510 Meta (room 926):10x/0.3 Plan Neofluar; 20x/0.75 Plan Apochromat; 40x/1.3 Plan Neofluar; 40x/1.2 C Apochromat; 63x/1.4 Plan Apochromat; 100X/1.3 Plan Neofluar.

LSM 780 (room 928):10x/0.45 Plan Apochromat; 20x/0.8 Plan Apochromat; 25x/0.8 LD LCI Plan Apochromat; 40x/1.2 C Apochromat; 40x/1.4 Plan Apochromat; 63x/1.4 Plan Apochromat.

Spinning Roper 2 cameras (room 924):10x/0.3 Plan Fluor; 20X/0.45 S Plan Fluor ELWD; 20x/0.75 Plan Fluor; 40x/0.6 S Plan Fluor ELWD; 100X/1.4 Plan Apo VC.

TEXTE

 

METROLOGY

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Cédric Matthews
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Cédric Matthews

Technical staff

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Elsa Auroux-castellani

Technical staff

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Technical staff

Training

Internal training :

  • Theoretical and practical training in Videomicroscopy, Confocal microscopy, Nonlinear Microscopy
  • Introduction to image processing with ImageJ software

External training, in collaboration with INSERM Marseille :

  • Videomicroscopy to confocal microscopy, concepts, practices and quality control
  • Image Processing with ImageJ, conceptual and practical bases for biology

External training, in collaboration with CNRS formation entreprises :

Find here all the training in all areas proposed by the CNRS formation entreprises

Useful infos

Application for interventions by equipment type

Contacts between users of the same equipment type