Platform

Electron microscopy

Scientific manager : P.F. Lenne
Technical manager : N. Brouilly

The Electron Microscopy department of the PICsL Imaging facility provides the scientific community with 1. the expertise, 2. the material and 3. electron microscopes necessary for molecular, cellular and tissue-level imaging by electron microscopy.

Our service offer comprises the sample preparation with the following methods: negative staining, plastic embedding, immuno-labelling, high pressure freezing, freeze substitution, (cryo)-ultramictotomy…

We routinely use advanced imaging modes such as electron tomography (ET), cryo-Electron Microscopy (cryo-EM), Scanning Transmission Electron Microscopy (STEM), Scanning Electron Microscopy in « Serial Block-Face » mode (see an example above), Correlative Light and Electron Microscopy (CLEM)…

For the users willing to work autonomously, we offer individual or group trainings for the conventional methods in transmission and scanning electron microscopy.

Please, do not hesitate to contact us: ibdm-plateau-microscopie(at)univ-amu.fr

 

PUBLICATION

Promoting Myelin Repair through In Vivo Neuroblast Reprogramming.

El Waly B, Cayre M, Durbec P.
Stem Cell Reports. 2018 Mar 27. pii: S2213-6711(18)30106-1. doi: 10.1016/j.stemcr.2018.02.015. PMID: 29606615

PUBLICATION

Tailoring drug release rates in hydrogel-based therapeutic delivery applications using graphene oxide

Puvirajesinghe TM, Zhi ZL, Craster RV, Guenneau S.
J R Soc Interface. 2018 Feb;15(139). pii: 20170949. doi: 10.1098/rsif.2017.0949. PMID: 29445040

PUBLICATION

Characterization and photodegradation mechanism of three Algerian wood species

Yasmina Ouadou, Djamel Aliouche, Marie-France Thevenon, Mohamed Djillali
Journal of Wood Science

PUBLICATION

Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness.

Leca J, Martinez S, Lac S, Nigri J, Secq V, Rubis M, Bressy C, Sergé A, Lavaut MN, Dusetti N, Loncle C, Roques J, Pietrasz D, Bousquet C, Garcia S, Granjeaud S, Ouaissi M, Bachet JB, Brun C, Iovanna JL, Zimmermann P, Vasseur S, Tomasini R.
J Clin Invest. 2016 Nov 1;126(11):4140-4156. doi: 10.1172/JCI87734. Epub 2016 Oct 4. PMID: 27701147

PUBLICATION

LAMP5 Fine-Tunes GABAergic Synaptic Transmission in Defined Circuits of the Mouse Brain.

Tiveron MC, Beurrier C, Céni C, Andriambao N, Combes A, Koehl M, Maurice N, Gatti E, Abrous DN, Kerkerian-Le Goff L, Pierre P, Cremer H.
PLoS One. 2016 Jun 7;11(6):e0157052. PMID: 27272053

PUBLICATION

Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution

Baati T, Al-Kattan A, Esteve MA, Njim L, Ryabchikov Y, Chaspoul F, Hammami M, Sentis M, Kabashin AV, Braguer D.
Sci Rep. 2016 May 6;6:25400. doi: 10.1038/srep25400. PMID: 27151839

PUBLICATION

PINK1-induced mitophagy promotes neuroprotection in Huntington's disease.

Khalil B, El Fissi N, Aouane A, Cabirol-Pol MJ, Rival T, Liévens JC.
Cell Death Dis. 2015 Jan 22;6:e1617. doi: 10.1038/cddis.2014.581. PMID: 25611391

PUBLICATION

Diversity of Magnetotactic Bacteria from a French Pristine Mediterranean Area.

Fuduche M, Postec A, Davidson S, Chauvin JP, Galès G, Hirschler-Réa A, Ollivier B, Wu LF, Pradel N.
Curr Microbiol. 2014 Dec 9. [Epub ahead of print] PMID: 25487117

PUBLICATION

Loss of Krox20 results in aortic valve regurgitation and impaired transcriptional activation of fibrillar collagen genes.

Odelin G, Faure E, Kober F, Maurel-Zaffran C, Théron A, Coulpier F, Guillet B, Bernard M, Avierinos JF, Charnay P, Topilko P, Zaffran S.
Cardiovasc Res. 2014 Dec 1;104(3):443-55. PMID: 25344368

PUBLICATION

TBX1 regulates epithelial polarity and dynamic basal filopodia in the second heart field.

Francou A, Saint-Michel E, Mesbah K, Kelly RG.
Development. 2014 Nov;141(22):4320-31. doi: 10.1242/dev.115022. PMID: 25371366

PUBLICATION

Repair of the injured spinal cord by implantation of a synthetic degradable block copolymer in rat.

Pertici V, Trimaille T, Laurin J, Felix MS, Marqueste T, Pettmann B, Chauvin JP, Gigmes D, Decherchi P.
Biomaterials. 2014 Aug;35(24):6248-58. PMID: 24814425

PUBLICATION

A dual role for planar cell polarity genes in ciliated cells

Boutin C, Labedan P, Dimidschstein J, Richard F, Cremer H, André P, Yang Y, Montcouquiol M, Goffinet AM, Tissir F.
Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):E3129-38. doi: 10.1073/pnas.1404988111. PMID: 25024228

PUBLICATION

CRB2 acts as a modifying factor of CRB1-related retinal dystrophies in mice.

Pellissier LP, Lundvig DM, Tanimoto N, Klooster J, Vos RM, Richard F, Sothilingam V, Garcia Garrido M, Le Bivic A, Seeliger MW, Wijnholds J.
Hum Mol Genet. 2014 Jul 15;23(14):3759-71. PMID: 24565864

PUBLICATION

Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology.

Legendre M, Bartoli J, Shmakova L, Jeudy S, Labadie K, Adrait A, Lescot M, Poirot O, Bertaux L, Bruley C, Couté Y, Rivkina E, Abergel C, Claverie JM.
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4274-9. PMID: 24591590

PUBLICATION

Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2.

Ghossoub R, Lembo F, Rubio A, Gaillard CB, Bouchet J, Vitale N, Slavík J, Machala M, Zimmermann P.
Nat Commun. 2014 Mar 18;5:3477. PMID: 24637612

PUBLICATION

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context.

Castillo-Briceno P, Kodjabachian L.
Front Zool. 2014 Feb 6;11(1):9. PMID: 24502321

PUBLICATION

Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection

Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O.
PLoS Pathog. 2014 Feb 20;10(2):e1003928. PMID: 24586151

PUBLICATION

Reversible lipid accumulation and associated division arrest of Mycobacterium avium in lipoprotein-induced foamy macrophages may resemble key events during latency and reactivation of tuberculosis.

Caire-Brändli I, Papadopoulos A, Malaga W, Marais D, Canaan S, Thilo L, de Chastellier C.
Infect Immun. 2014 Feb;82(2):476-90. PMID: 24478064

PUBLICATION

Drebrin E depletion in human intestinal epithelial cells mimics Rab8a loss of function.

Vacca B, Bazellières E, Nouar R, Harada A, Massey-Harroche D, Le Bivic A.
Hum Mol Genet. 2014 Jan 12. PMID: 24399445

PUBLICATION

Regulation of mycolactone, the Mycobacterium ulcerans toxin, depends on nutrient source.

Deshayes C, Angala SK, Marion E, Brandli I, Babonneau J, Preisser L, Eyangoh S, Delneste Y, Legras P, De Chastellier C, Stinear TP, Jackson M, Marsollier L.
PLoS Negl Trop Dis. 2013 Nov 14;7(11):e2502. PMID: 24244764

PUBLICATION

Pandoraviruses: amoeba viruses with genomes up to 2.5 Mb reaching that of parasitic eukaryotes.

Philippe N, Legendre M, Doutre G, Couté Y, Poirot O, Lescot M, Arslan D, Seltzer V, Bertaux L, Bruley C, Garin J, Claverie JM, Abergel C.
Science. 2013 Jul 19;341(6143):281-6. PMID: 23869018

PUBLICATION

Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes.

Santini S, Jeudy S, Bartoli J, Poirot O, Lescot M, Abergel C, Barbe V, Wommack KE, Noordeloos AA, Brussaard CP, Claverie JM.
Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10800-5. PMID: 23754393

PUBLICATION

Pathogenic brucellae replicate in human trophoblasts.

Salcedo SP, Chevrier N, Lacerda TL, Ben Amara A, Gerart S, Gorvel VA, de Chastellier C, Blasco JM, Mege JL, Gorvel JP.
J Infect Dis. 2013 Apr;207(7):1075-83. PMID: 23303808

PUBLICATION

Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene.

Alves CH, Sanz AS, Park B, Pellissier LP, Tanimoto N, Beck SC, Huber G, Murtaza M, Richard F, Sridevi Gurubaran I, Garcia Garrido M, Levelt CN, Rashbass P, Le Bivic A, Seeliger MW, Wijnholds J.
Hum Mol Genet. 2013 Jan 1;22(1):35-50. PMID: 23001562

PUBLICATION

Structure of the dimeric RC-LH1-PufX complex from Rhodobaca bogoriensis investigated by electron microscopy.

Semchonok DA, Chauvin JP, Frese RN, Jungas C, Boekema EJ.
Philos Trans R Soc Lond B Biol Sci. 2012 Dec 19 PMID: 23148268

PUBLICATION

Phosphorylation of mycobacterial PcaA inhibits mycolic acid cyclopropanation: consequences for intracellular survival and for phagosome maturation block.

Corrales RM, Molle V, Leiba J, Mourey L, de Chastellier C, Kremer L.
J Biol Chem. 2012 Jul 27;287(31):26187-99. PMID: 22621931

PUBLICATION

Metallobiology of host-pathogen interactions: an intoxicating new insight.

Botella H, Stadthagen G, Lugo-Villarino G, de Chastellier C, Neyrolles O.
Trends Microbiol. 2012 Mar;20(3):106-12. PMID: 22305804

PUBLICATION

Olesoxime accelerates myelination and promotes repair in models of demyelination.

Magalon K, Zimmer C, Cayre M, Khaldi J, Bourbon C, Robles I, Tardif G, Viola A, Pruss RM, Bordet T, Durbec P.
Ann Neurol. 2012 Feb;71(2):213-26. PMID: 22367994

PUBLICATION

[A novel role for transition metals in anti-microbial immunity].

Botella H, Stadthagen G, de Chastellier C, Neyrolles O.
Med Sci (Paris). 2012 Jan;28(1):18-21. PMID: 22289820

PUBLICATION

Hippocampal GABAergic neurons are susceptible to amyloid-β toxicity in vitro and are decreased in number in the Alzheimer's disease TgCRND8 mouse model.

Krantic S, Isorce N, Mechawar N, Davoli MA, Vignault E, Albuquerque M, Chabot JG, Moyse E, Chauvin JP, Aubert I, McLaurin J, Quirion R.
J Alzheimers Dis. 2012;29(2):293-308. PMID: 22232004

PUBLICATION

Mycobacterial p(1)-type ATPases mediate resistance to zinc poisoning in human macrophages.

Botella H, Peyron P, Levillain F, Poincloux R, Poquet Y, Brandli I, Wang C, Tailleux L, Tilleul S, Charrière GM, Waddell SJ, Foti M, Lugo-Villarino G, Gao Q, Maridonneau-Parini I, Butcher PD, Castagnoli PR, Gicquel B, de Chastellier C, Neyrolles O.
Cell Host Microbe. 2011 Sep 15;10(3):248-59. PMID: 21925112

PUBLICATION

Body plan innovation in treehoppers through the evolution of an extra wing-like appendage.

Prud'homme B, Minervino C, Hocine M, Cande JD, Aouane A, Dufour HD, Kassner VA, Gompel N.
Nature. 2011 May 5;473(7345):83-6. PMID: 21544145

PUBLICATION

Trafficking and secretion of matrix metalloproteinase-2 in olfactory ensheathing glial cells: A role in cell migration?

Gueye Y, Ferhat L, Sbai O, Bianco J, Ould-Yahoui A, Bernard A, Charrat E, Chauvin JP, Risso JJ, Féron F, Rivera S, Khrestchatisky M.
Glia. 2011 May;59(5):750-70. PMID: 21360755

PUBLICATION

Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice.

Ganay T, Boizot A, Burrer R, Chauvin JP, Bomont P.
Mol Neurodegener. 2011 Apr 12;6:25. PMID: 21486449

PUBLICATION

Inner-membrane proteins PMI/TMEM11 regulate mitochondrial morphogenesis independently of the DRP1/MFN fission/fusion pathways.

Rival T, Macchi M, Arnauné-Pelloquin L, Poidevin M, Maillet F, Richard F, Fatmi A, Belenguer P, Royet J.
EMBO Rep. 2011 Mar;12(3):223-30. PMID: 21274005

PUBLICATION

Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.

Nisoli I, Chauvin JP, Napoletano F, Calamita P, Zanin V, Fanto M, Charroux B.
Cell Death Differ. 2010 Oct;17(10):1577-87. PMID: 20339376

PUBLICATION

The virulence protein SopD2 regulates membrane dynamics of Salmonella-containing vacuoles.

Schroeder N, Henry T, de Chastellier C, Zhao W, Guilhon AA, Gorvel JP, Méresse S.
PLoS Pathog. 2010 Jul 15;6(7):e1001002 PMID: 20664790

PUBLICATION

Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein.

Sedjaï F, Acquaviva C, Chevrier V, Chauvin JP, Coppin E, Aouane A, Coulier F, Tolun A, Pierres M, Birnbaum D, Rosnet O.
J Cell Sci. 2010 Jul 15;123(Pt 14):2391-401. PMID: 20551181

PUBLICATION

A polycystin-2 (TRPP2) dimerization domain essential for the function of heteromeric polycystin complexes.

Giamarchi A, Feng S, Rodat-Despoix L, Xu Y, Bubenshchikova E, Newby LJ, Hao J, Gaudioso C, Crest M, Lupas AN, Honoré E, Williamson MP, Obara T, Ong AC, Delmas P.
EMBO J. 2010 Apr 7;29(7):1176-91. doi: 10.1038/emboj.2010.18. PMID: 20168298

PUBLICATION

Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.

Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M.
Glia. 2010 Feb;58(3):344-66. PMID: 19780201

PUBLICATION

GABA action in immature neocortical neurons directly depends on the availability of ketone bodies.

Rheims S, Holmgren CD, Chazal G, Mulder J, Harkany T, Zilberter T, Zilberter Y.
J Neurochem. 2009 Aug;110(4):1330-8. PMID: 19558450

PUBLICATION

The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication.

Fugier E, Salcedo SP, de Chastellier C, Pophillat M, Muller A, Arce-Gorvel V, Fourquet P, Gorvel JP.
PLoS Pathog. 2009 Jun;5(6):e1000487. PMID: 19557163

PUBLICATION

PSD95beta regulates plasma membrane Ca2+ pump localization at the photoreceptor synapse.

Aartsen WM, Arsanto JP, Chauvin JP, Vos RM, Versteeg I, Cardozo BN, Bivic AL, Wijnholds J.
Mol Cell Neurosci. 2009 Jun;41(2):156-65. PMID: 19233278

PUBLICATION

The centrosomal FOP protein is required for cell cycle progression and survival.

Acquaviva C, Chevrier V, Chauvin JP, Fournier G, Birnbaum D, Rosnet O.
Cell Cycle. 2009 Apr 15;8(8):1217-27. PMID: 19305129

PUBLICATION

Ajuba: a new microtubule-associated protein that interacts with BUBR1 and Aurora B at kinetochores in metaphase.

Ferrand A, Chevrier V, Chauvin JP, Birnbaum D.
Biol Cell. 2009 Apr;101(4):221-35. PMID: 18710370

PUBLICATION

Vesicular trafficking and secretion of matrix metalloproteinases-2, -9 and tissue inhibitor of metalloproteinases-1 in neuronal cells.

Sbai O, Ferhat L, Bernard A, Gueye Y, Ould-Yahoui A, Thiolloy S, Charrat E, Charton G, Tremblay E, Risso JJ, Chauvin JP, Arsanto JP, Rivera S, Khrestchatisky M.
Mol Cell Neurosci. 2008 Dec;39(4):549-68. PMID: 18817873

PUBLICATION

Foamy macrophages from tuberculous patients' granulomas constitute a nutrient-rich reservoir for M. tuberculosis persistence.

Peyron P, Vaubourgeix J, Poquet Y, Levillain F, Botanch C, Bardou F, Daffé M, Emile JF, Marchou B, Cardona PJ, de Chastellier C, Altare F.
PLoS Pathog. 2008 Nov;4(11):e1000204. PMID: 19002241

PUBLICATION

Marked mitochondrial alterations upon starvation without cell death, caspases or Bcl-2 family members.

Kosta A, Luciani MF, Geerts WJ, Golstein P.
Biochim Biophys Acta. 2008 Oct;1783(10):2013-9. PMID: 18652851

PUBLICATION

Functional role of the PE domain and immunogenicity of the Mycobacterium tuberculosis triacylglycerol hydrolase LipY.

Mishra KC, de Chastellier C, Narayana Y, Bifani P, Brown AK, Besra GS, Katoch VM, Joshi B, Balaji KN, Kremer L.
Infect Immun. 2008 Jan;76(1):127-40. PMID: 17938218

PUBLICATION

Analysis of autophagic and necrotic cell death in Dictyostelium.

Giusti C, Kosta A, Lam D, Tresse E, Luciani MF, Golstein P.
Methods Enzymol. 2008;446:1-15. PMID: 18603113

PUBLICATION

Autophagy and autophagic cell death in Dictyostelium.

Tresse E, Giusti C, Kosta A, Luciani MF, Golstein P.
Methods Enzymol. 2008;451:343-58. PMID: 19185731

PUBLICATION

From autophagic to necrotic cell death in Dictyostelium.

Tresse E, Kosta A, Luciani MF, Golstein P.
Semin Cancer Biol. 2007 Apr;17(2):94-100. PMID: 17150370

PUBLICATION

Birc1e/Naip5 rapidly antagonizes modulation of phagosome maturation by Legionella pneumophila.

Fortier A, de Chastellier C, Balor S, Gros P.
Cell Microbiol. 2007 Apr;9(4):910-23. PMID: 17087731

PUBLICATION

Nkx2.5 cell-autonomous gene function is required for the postnatal formation of the peripheral ventricular conduction system.

Meysen S, Marger L, Hewett KW, Jarry-Guichard T, Agarkova I, Chauvin JP, Perriard JC, Izumo S, Gourdie RG, Mangoni ME, Nargeot J, Gros D, Miquerol L.
Dev Biol. 2007 Mar 15;303(2):740-53. PMID: 17250822

PUBLICATION

Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo.

Miranda-Tello E, Fardeau ML, Joulian C, Magot M, Thomas P, Tholozan JL, Ollivier B.
Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):40-4. PMID: 17220438

PUBLICATION

Atrophin contributes to the negative regulation of epidermal growth factor receptor signaling in Drosophila.

Charroux B, Freeman M, Kerridge S, Baonza A.
Dev Biol. 2006 Mar 15;291(2):278-90. PMID: 16445904

PUBLICATION

Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.

Lapaque N, Forquet F, de Chastellier C, Mishal Z, Jolly G, Moreno E, Moriyon I, Heuser JE, He HT, Gorvel JP.
Cell Microbiol. 2006 Feb;8(2):197-206. PMID: 16441431

PUBLICATION

Cholesterol depletion in Mycobacterium avium-infected macrophages overcomes the block in phagosome maturation and leads to the reversible sequestration of viable mycobacteria in phagolysosome-derived autophagic vacuoles.

de Chastellier C, Thilo L.
Cell Microbiol. 2006 Feb;8(2):242-56. PMID: 16441435

PUBLICATION

Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation.

Pilot F, Philippe JM, Lemmers C, Chauvin JP, Lecuit T.
Development. 2006 Feb;133(4):711-23. PMID: 16421189

PUBLICATION

Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.

Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S.
Cell. 2005 Oct 7;123(1):157-70. PMID: 16213220

PUBLICATION

Identification and structural characterization of an unusual mycobacterial monomeromycolyl-diacylglycerol.

Kremer L, de Chastellier C, Dobson G, Gibson KJ, Bifani P, Balor S, Gorvel JP, Locht C, Minnikin DE, Besra GS.
Mol Microbiol. 2005 Aug;57(4):1113-26. PMID: 16091048

PUBLICATION

mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone.

Nieoullon V, Belvindrah R, Rougon G, Chazal G.
Mol Cell Neurosci. 2005 Mar;28(3):462-74. PMID: 15737737

PUBLICATION

Expanded polyglutamine peptides disrupt EGF receptor signaling and glutamate transporter expression in Drosophila.

Liévens JC1, Rival T, Iché M, Chneiweiss H, Birman S.
Hum Mol Genet. 2005 Mar 1;14(5):713-24. PMID: 15677486

PUBLICATION

Bacteria spurned by self-absorbed cells.

Gorvel JP, de Chastellier C.
Nat Med. 2005 Jan;11(1):18-9. PMID: 15635436

PUBLICATION

Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.

Kosta A, Roisin-Bouffay C, Luciani MF, Otto GP, Kessin RH, Golstein P.
J Biol Chem. 2004 Nov 12;279(46):48404-9. PMID: 15358773

PUBLICATION

Mycobacterium tuberculosis and Mycobacterium avium modify the composition of the phagosomal membrane in infected macrophages by selective depletion of cell surface-derived glycoconjugates.

Pietersen R, Thilo L, de Chastellier C.
Eur J Cell Biol. 2004 May;83(4):153-8. PMID: 15260437

PUBLICATION

Petrotoga mexicana sp. nov., a novel thermophilic, anaerobic and xylanolytic bacterium isolated from an oil-producing well in the Gulf of Mexico.

Miranda-Tello E, Fardeau ML, Thomas P, Ramirez F, Casalot L, Cayol JL, Garcia JL, Ollivier B.
Int J Syst Evol Microbiol. 2004 Jan;54(Pt 1):169-74. PMID: 14742476

PUBLICATION

Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis.

Pelissier A, Chauvin JP, Lecuit T.
Curr Biol. 2003 Oct 28;13(21):1848-57. PMID: 14588240

PUBLICATION

Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum.

Celli J, de Chastellier C, Franchini DM, Pizarro-Cerda J, Moreno E, Gorvel JP.
J Exp Med. 2003 Aug 18;198(4):545-56. PMID: 12925673

PUBLICATION

Production of a polyunsaturated isoprenoid wax ester during aerobic metabolism of squalene by Marinobacter squalenivorans sp. nov.

Rontani JF, Mouzdahir A, Michotey V, Caumette P, Bonin P.
Appl Environ Microbiol. 2003 Jul;69(7):4167-76. PMID: 12839795

PUBLICATION

The type 3 inositol 1,4,5-trisphosphate receptor is concentrated at the tight junction level in polarized MDCK cells.

Colosetti P, Tunwell RE, Cruttwell C, Arsanto JP, Mauger JP, Cassio D.
J Cell Sci. 2003 Jul 1;116(Pt 13):2791-803. PMID: 12759372

PUBLICATION

Dictyostelium cell death: early emergence and demise of highly polarized paddle cells.

Levraud JP, Adam M, Luciani MF, de Chastellier C, Blanton RL, Golstein P.
J Cell Biol. 2003 Mar 31;160(7):1105-14. PMID: 12654899

PUBLICATION

Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.

Soustelle L, Besson MT, Rival T, Birman S.
Dev Biol. 2002 Aug 15;248(2):294-306. PMID: 12167405

PUBLICATION

Pericardin, a Drosophila type IV collagen-like protein is involved in the morphogenesis and maintenance of the heart epithelium during dorsal ectoderm closure.

Chartier A, Zaffran S, Astier M, Sémériva M, Gratecos D.
Development. 2002 Jul;129(13):3241-53. PMID: 12070098

PUBLICATION

Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure.

Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
J Neurosci Methods. 2002 May 30;117(1):81-5. PMID: 12084567

PUBLICATION

Increased neurogenesis in adult mCD24-deficient mice.

Belvindrah R, Rougon G, Chazal G.
J Neurosci. 2002 May 1;22(9):3594-607. PMID: 11978835

PUBLICATION

TCR signal initiation machinery is pre-assembled and activated in a subset of membrane rafts.

Drevot P, Langlet C, Guo XJ, Bernard AM, Colard O, Chauvin JP, Lasserre R, He HT.
EMBO J. 2002 Apr 15;21(8):1899-908. PMID: 11953309

PUBLICATION

PrP expression and replication by Schwann cells: implications in prion spreading.

Follet J, Lemaire-Vieille C, Blanquet-Grossard F, Podevin-Dimster V, Lehmann S, Chauvin JP, Decavel JP, Varea R, Grassi J, Fontès M, Cesbron JY.
J Virol. 2002 Mar;76(5):2434-9. PMID: 11836421

microscope1Transmission Electron
Microscope FEI Tecnai G2 200 kV.

This microscope is designed for advanced imaging modes such as electron tomography, STEM and cryo-electron microscopy. With its 200kV gun and the optical quality of its column, it can reach a magnification of about 200 000x. It is equipped with an Olympus Veleta 2k camera and an FEI Eagle 4k camera.

This microscope has been co-funded by the Plan Cancer, the INSB, the FEDER and the IBDM.

 

microscope2Scanning Electron
Microscope FEI Teneo VS.

With this microscope, our users can aqcuire conventional MEB images of an outstanding quality. Moreover, this microscope is equipped with an automatized ultra-microtomy module. By the technique of Serial Block-Face, the microscope autonomously alternate between cutting and imaging steps of an embedded sample. With a rate of one image per minute, this microscope is a revolution in the field of 3D electron microscopy. For instance, given a field of view of 40µm width and a pixel size of 5x5x40 nm, the microscope can image 20µm in depth within the sample in an overnight acquisition.

This microscope has been co-funded by the Bettencourt Schueller Society, the GIS IBISA, the Labex INFORM, the PACA area, Aix-Marseille University and the Plan Cancer.

 

microscope3Transmission Electron
Microscope FEI Morgagni 120 kV.

This microscope enables the insertion of samples in less than 20 seconds. It is therefore very useful for grids quality checks. It is equipped with a camera and can be used to acquire images up to a 20 000x magnification.

 

Sample preparation devices

  • Leica EMPact 2, high pressure freezing machine
  • Leica AFS 2, freeze substitution apparatus
  • Leica AFS, freeze substitution apparatus
  • Leica EM AMW, sample preparation automate
  • Leica UC7, (cryo)-ultramicrotome
  • Leica UCT, (cryo)-ultramicrotome
  • LKB 7801A, knife maker
  • Balzers CPD030, critical point dryer
  • Edwards S150B, glow discharge and sputter coater apparatus

 

Funding

display123

Bettancourt

inform

IBISA

aix-mars

region

cnrs

cancer

Member of

FBI

Members more

Aïcha Aouane Fabrice Richard
Nicolas Brouilly
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Nicolas Brouilly

Technical staff

Aïcha Aouane
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Aïcha Aouane

Technical staff

Fabrice Richard
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Fabrice Richard

Technical staff

Useful infos

For a map to find us on the Luminy campus, click here

The pricing of the microscopes booking, our service offer and our training offer is based on fares validated by the CNRS.

Since 2006, the facility staff works accordingly to a quality approach defined by the ISO 9001 standard.