Platform
Electron microscopy
Scientific manager :
P.F. Lenne
Technical manager :
N. Brouilly
The Electron Microscopy department of the PICsL Imaging facility provides the scientific community with 1. the expertise, 2. the material and 3. electron microscopes necessary for molecular, cellular and tissue-level imaging by electron microscopy.
Our service offer comprises the sample preparation with the following methods: negative staining, plastic embedding, immuno-labelling, high pressure freezing, freeze substitution, (cryo)-ultramictotomy…
We routinely use advanced imaging modes such as electron tomography (ET), Scanning Transmission Electron Microscopy (STEM), Scanning Electron Microscopy in « Serial Block-Face » mode (see an example above), Correlative Light and Electron Microscopy (CLEM)…
For the users willing to work autonomously, we offer individual or group trainings for the conventional methods in transmission and scanning electron microscopy.
Please, do not hesitate to contact us: ibdm-plateau-microscopie(at)univ-amu.fr
PUBLICATION
October 1st, 2021
NUPR1 inhibitor ZZW-115 induces ferroptosis in a mitochondria-dependent manner
PUBLICATION
September 1st, 2021
Expanding the Occurrence of Polysaccharides to the Viral World: The Case of Mimivirus
PUBLICATION
August 21st, 2021
Evolution of mechanisms controlling epithelial morphogenesis across animals: new insights from dissociation-reaggregation experiments in the sponge Oscarella lobularis
PUBLICATION
August 16th, 2021
Assessment of Pharmacokinetics, Toxicity, and Biodistribution of a High Dose of Titanate Nanotubes Following Intravenous Injection in Mice: A Promising Nanosystem of Medical Interest
PUBLICATION
July 20th, 2021
Human and Animal Dirofilariasis in Southeast of France
PUBLICATION
July 13th, 2021
DNAse-dependent, NET-independent pathway of thrombus formation in vivo
PUBLICATION
May 17th, 2021
ppGpp influences protein protection, growth and photosynthesis in Phaeodactylum tricornutum
PUBLICATION
May 8th, 2021
TP53INP1 exerts neuroprotection under ageing and Parkinson's disease-related stress condition
PUBLICATION
April 7th, 2021
Myofibril and mitochondria morphogenesis are coordinated by a mechanical feedback mechanism in muscle
PUBLICATION
March 31st, 2021
Characterization of Mollivirus kamchatka, the First Modern Representative of the Proposed Molliviridae Family of Giant Viruses
PUBLICATION
March 11th, 2021
Influence of force volume indentation parameters and processing method in wood cell walls nanomechanical studies
PUBLICATION
December 17th, 2020
GABA-glutamate supramammillary neurons control theta and gamma oscillations in the dentate gyrus during paradoxical (REM) sleep
PUBLICATION
December 12th, 2020
Marine Bacteria Display Different Escape Mechanisms When Facing Their Protozoan Predators
PUBLICATION
December 4th, 2020
Responses to the Hydrostatic Pressure of Surface and Subsurface Strains of Pseudothermotoga elfii Revealing the Piezophilic Nature of the Strain Originating From an Oil-Producing Well
PUBLICATION
November 17th, 2020
Targeting Mitochondrial Complex I Overcomes Chemoresistance in High OXPHOS Pancreatic Cancer
PUBLICATION
November 9th, 2020
Terahertz probing of sunflower leaf multilayer organization
PUBLICATION
October 13th, 2020
Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites
PUBLICATION
October 1st, 2020
Mitochondrial morphology and activity regulate furrow ingression and contractile ring dynamics in Drosophila cellularization
PUBLICATION
July 14th, 2020
Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages
PUBLICATION
June 15th, 2020
Human ZKSCAN3 and Drosophila M1BP are functionally homologous transcription factors in autophagy regulation
PUBLICATION
March 16th, 2020
A fungal family of lytic polysaccharide monooxygenase-like copper proteins
PUBLICATION
February 27th, 2020
Fabrication of sharp silicon arrays to wound Caenorhabditis elegans
PUBLICATION
December 10th, 2019
Exploration of the Propagation of Transpovirons Within Mimiviridae Reveals a Unique Example of Commensalism in the Viral World
PUBLICATION
November 26th, 2019
Dissecting the Anticancer Mechanism of Trifluoperazine on Pancreatic Ductal Adenocarcinoma
PUBLICATION
November 20th, 2019
Receptor-specific Interactome as a Hub for Rapid Cue-Induced Selective Translation in Axons
PUBLICATION
November 14th, 2019
The Interaction of Platelets With Colorectal Cancer Cells Inhibits Tumor Growth but Promotes Metastasis
PUBLICATION
October 1st, 2019
Amino acids promote black carbon aggregation and microbial colonization in coastal waters off Vietnam
PUBLICATION
September 9th, 2019
In Vivo Evaluation of Safety, Biodistribution and Pharmacokinetics of Laser-Synthesized Gold Nanoparticles
PUBLICATION
August 16th, 2019
Correlative Single-Molecule Localization Microscopy and Electron Tomography Reveals Endosome Nanoscale Domains
PUBLICATION
December 3rd, 2018
The conduit system exports locally secreted IgM from lymph nodes
PUBLICATION
November 19th, 2018
Inactivation of NUPR1 promotes cell death by coupling ER-stress responses with necrosis
PUBLICATION
November 7th, 2018
CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.
PUBLICATION
August 22nd, 2018
Delineating the Physiological Roles of the PE and Catalytic Domains of LipY in Lipid Consumption in Mycobacterium-Infected Foamy Macrophages
PUBLICATION
August 19th, 2018
Mitofusin gain and loss of function drive pathogenesis in Drosophila models of CMT2A neuropathy
PUBLICATION
August 1st, 2018
Correct Laminar Positioning in the Neocortex Influences Proper Dendritic and Synaptic Development
PUBLICATION
July 23rd, 2018
Vnn1 pantetheinase limits the Warburg effect and sarcoma growth by rescuing mitochondrial activity
PUBLICATION
May 21st, 2018
Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis
PUBLICATION
March 27th, 2018
Promoting Myelin Repair through In Vivo Neuroblast Reprogramming.
PUBLICATION
March 5th, 2018
Nanoplanktonic diatoms are globally overlooked but play a role in spring blooms and carbon export
PUBLICATION
February 15th, 2018
Tailoring drug release rates in hydrogel-based therapeutic delivery applications using graphene oxide
PUBLICATION
June 19th, 2017
Characterization and photodegradation mechanism of three Algerian wood species
PUBLICATION
March 30th, 2017
Epithelial tension in the second heart field promotes mouse heart tube elongation.
PUBLICATION
January 13th, 2017
Soluble Ectodomain CD163 and Extracellular Vesicle-Associated CD163 Are Two Differently Regulated Forms of 'Soluble CD163' in Plasma
PUBLICATION
November 6th, 2016
The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages
PUBLICATION
November 5th, 2016
MAB_3551c encodes the primary triacylglycerol synthase involved in lipid accumulation in Mycobacterium abscessus
PUBLICATION
November 1st, 2016
Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness.
PUBLICATION
October 7th, 2016
Experimental Models of Foamy Macrophages and Approaches for Dissecting the Mechanisms of Lipid Accumulation and Consumption during Dormancy and Reactivation of Tuberculosis
PUBLICATION
September 2nd, 2016
Biochemical and Spectroscopic Characterization of Highly Stable Photosystem II Supercomplexes from Arabidopsis
PUBLICATION
August 1st, 2016
Deciphering the adaptation strategies of Desulfovibrio piezophilus to hydrostatic pressure through metabolic and transcriptional analyses
PUBLICATION
June 7th, 2016
LAMP5 Fine-Tunes GABAergic Synaptic Transmission in Defined Circuits of the Mouse Brain.
PUBLICATION
May 6th, 2016
Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution
PUBLICATION
March 5th, 2016
Insights into the smooth-to-rough transitioning in Mycobacterium bolletii unravels a functional Tyr residue conserved in all mycobacterial MmpL family members
PUBLICATION
November 25th, 2015
Brucella discriminates between mouse dendritic cell subsets upon in vitro infection
PUBLICATION
October 10th, 2015
Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis
PUBLICATION
June 7th, 2015
Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1
PUBLICATION
January 22nd, 2015
PINK1-induced mitophagy promotes neuroprotection in Huntington's disease.
PUBLICATION
December 9th, 2014
Diversity of Magnetotactic Bacteria from a French Pristine Mediterranean Area.
PUBLICATION
December 1st, 2014
Loss of Krox20 results in aortic valve regurgitation and impaired transcriptional activation of fibrillar collagen genes.
PUBLICATION
November 1st, 2014
TBX1 regulates epithelial polarity and dynamic basal filopodia in the second heart field.
PUBLICATION
August 1st, 2014
Repair of the injured spinal cord by implantation of a synthetic degradable block copolymer in rat.
PUBLICATION
July 29th, 2014
A dual role for planar cell polarity genes in ciliated cells
PUBLICATION
July 15th, 2014
CRB2 acts as a modifying factor of CRB1-related retinal dystrophies in mice.
PUBLICATION
March 18th, 2014
Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology.
PUBLICATION
March 18th, 2014
Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2.
PUBLICATION
February 6th, 2014
Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context.
PUBLICATION
February 1st, 2014
Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection
PUBLICATION
February 1st, 2014
Reversible lipid accumulation and associated division arrest of Mycobacterium avium in lipoprotein-induced foamy macrophages may resemble key events during latency and reactivation of tuberculosis.
PUBLICATION
January 12th, 2014
Drebrin E depletion in human intestinal epithelial cells mimics Rab8a loss of function.
PUBLICATION
November 14th, 2013
Regulation of mycolactone, the Mycobacterium ulcerans toxin, depends on nutrient source.
PUBLICATION
September 27th, 2013
Pandoraviruses: amoeba viruses with genomes up to 2.5 Mb reaching that of parasitic eukaryotes.
PUBLICATION
June 25th, 2013
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes.
PUBLICATION
April 1st, 2013
Pathogenic brucellae replicate in human trophoblasts.
PUBLICATION
January 1st, 2013
Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene.
PUBLICATION
December 19th, 2012
Structure of the dimeric RC-LH1-PufX complex from Rhodobaca bogoriensis investigated by electron microscopy.
PUBLICATION
July 27th, 2012
Phosphorylation of mycobacterial PcaA inhibits mycolic acid cyclopropanation: consequences for intracellular survival and for phagosome maturation block.
PUBLICATION
March 20th, 2012
Metallobiology of host-pathogen interactions: an intoxicating new insight.
PUBLICATION
February 1st, 2012
Olesoxime accelerates myelination and promotes repair in models of demyelination.
PUBLICATION
January 28th, 2012
[A novel role for transition metals in anti-microbial immunity].
PUBLICATION
January 1st, 2012
Hippocampal GABAergic neurons are susceptible to amyloid-β toxicity in vitro and are decreased in number in the Alzheimer's disease TgCRND8 mouse model.
PUBLICATION
September 15th, 2011
Mycobacterial p(1)-type ATPases mediate resistance to zinc poisoning in human macrophages.
PUBLICATION
June 5th, 2011
Body plan innovation in treehoppers through the evolution of an extra wing-like appendage.
PUBLICATION
May 1st, 2011
Trafficking and secretion of matrix metalloproteinase-2 in olfactory ensheathing glial cells: A role in cell migration?
PUBLICATION
April 15th, 2011
Neuronal chloride accumulation and excitatory GABA underlie aggravation of neonatal epileptiform activities by phenobarbital
PUBLICATION
April 12th, 2011
Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice.
PUBLICATION
March 1st, 2011
Inner-membrane proteins PMI/TMEM11 regulate mitochondrial morphogenesis independently of the DRP1/MFN fission/fusion pathways.
PUBLICATION
October 1st, 2010
Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.
PUBLICATION
July 15th, 2010
The virulence protein SopD2 regulates membrane dynamics of Salmonella-containing vacuoles.
PUBLICATION
July 15th, 2010
Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein.
PUBLICATION
April 7th, 2010
A polycystin-2 (TRPP2) dimerization domain essential for the function of heteromeric polycystin complexes.
PUBLICATION
February 1st, 2010
Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.
PUBLICATION
August 11th, 2009
GABA action in immature neocortical neurons directly depends on the availability of ketone bodies.
PUBLICATION
June 5th, 2009
The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication.
PUBLICATION
June 1st, 2009
PSD95beta regulates plasma membrane Ca2+ pump localization at the photoreceptor synapse.
PUBLICATION
April 15th, 2009
The centrosomal FOP protein is required for cell cycle progression and survival.
PUBLICATION
April 1st, 2009
Ajuba: a new microtubule-associated protein that interacts with BUBR1 and Aurora B at kinetochores in metaphase.
PUBLICATION
December 1st, 2008
Vesicular trafficking and secretion of matrix metalloproteinases-2, -9 and tissue inhibitor of metalloproteinases-1 in neuronal cells.
PUBLICATION
November 4th, 2008
Foamy macrophages from tuberculous patients' granulomas constitute a nutrient-rich reservoir for M. tuberculosis persistence.
PUBLICATION
October 1st, 2008
Marked mitochondrial alterations upon starvation without cell death, caspases or Bcl-2 family members.
PUBLICATION
January 1st, 2008
Functional role of the PE domain and immunogenicity of the Mycobacterium tuberculosis triacylglycerol hydrolase LipY.
PUBLICATION
January 1st, 2008
Analysis of autophagic and necrotic cell death in Dictyostelium.
PUBLICATION
January 1st, 2008
Autophagy and autophagic cell death in Dictyostelium.
PUBLICATION
April 17th, 2007
From autophagic to necrotic cell death in Dictyostelium.
PUBLICATION
April 9th, 2007
Birc1e/Naip5 rapidly antagonizes modulation of phagosome maturation by Legionella pneumophila.
PUBLICATION
March 15th, 2007
Nkx2.5 cell-autonomous gene function is required for the postnatal formation of the peripheral ventricular conduction system.
PUBLICATION
January 1st, 2007
Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo.
PUBLICATION
March 15th, 2006
Atrophin contributes to the negative regulation of epidermal growth factor receptor signaling in Drosophila.
PUBLICATION
February 8th, 2006
Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.
PUBLICATION
February 8th, 2006
Cholesterol depletion in Mycobacterium avium-infected macrophages overcomes the block in phagosome maturation and leads to the reversible sequestration of viable mycobacteria in phagolysosome-derived autophagic vacuoles.
PUBLICATION
February 1st, 2006
Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation.
PUBLICATION
October 7th, 2005
Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.
PUBLICATION
August 1st, 2005
Identification and structural characterization of an unusual mycobacterial monomeromycolyl-diacylglycerol.
PUBLICATION
March 28th, 2005
mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone.
PUBLICATION
March 1st, 2005
Expanded polyglutamine peptides disrupt EGF receptor signaling and glutamate transporter expression in Drosophila.
PUBLICATION
January 1st, 2005
Bacteria spurned by self-absorbed cells.
PUBLICATION
November 12th, 2004
Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.
PUBLICATION
May 1st, 2004
Mycobacterium tuberculosis and Mycobacterium avium modify the composition of the phagosomal membrane in infected macrophages by selective depletion of cell surface-derived glycoconjugates.
PUBLICATION
January 1st, 2004
Petrotoga mexicana sp. nov., a novel thermophilic, anaerobic and xylanolytic bacterium isolated from an oil-producing well in the Gulf of Mexico.
PUBLICATION
October 28th, 2003
Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis.
PUBLICATION
August 18th, 2003
Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum.
PUBLICATION
July 1st, 2003
Production of a polyunsaturated isoprenoid wax ester during aerobic metabolism of squalene by Marinobacter squalenivorans sp. nov.
PUBLICATION
July 1st, 2003
The type 3 inositol 1,4,5-trisphosphate receptor is concentrated at the tight junction level in polarized MDCK cells.
PUBLICATION
March 31st, 2003
Dictyostelium cell death: early emergence and demise of highly polarized paddle cells.
PUBLICATION
August 15th, 2002
Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.
PUBLICATION
July 1st, 2002
Pericardin, a Drosophila type IV collagen-like protein is involved in the morphogenesis and maintenance of the heart epithelium during dorsal ectoderm closure.
PUBLICATION
May 30th, 2002
Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure.
PUBLICATION
May 1st, 2002
Increased neurogenesis in adult mCD24-deficient mice.
PUBLICATION
April 15th, 2002
TCR signal initiation machinery is pre-assembled and activated in a subset of membrane rafts.
PUBLICATION
March 1st, 2002
PrP expression and replication by Schwann cells: implications in prion spreading.
Transmission Electron
Microscope FEI Tecnai G2 200 kV.
This microscope is designed for advanced imaging modes such as electron tomography, STEM and cryo-electron microscopy. With its 200kV gun and the optical quality of its column, it can reach a magnification of about 200 000x. It is equipped with an Olympus Veleta 2k camera and an FEI Eagle 4k camera.
This microscope has been co-funded by the Plan Cancer, the INSB, the FEDER and the IBDM.
Scanning Electron
Microscope FEI Teneo VS.
With this microscope, our users can aqcuire conventional MEB images of an outstanding quality. Moreover, this microscope is equipped with an automatized ultra-microtomy module. By the technique of Serial Block-Face, the microscope autonomously alternate between cutting and imaging steps of an embedded sample. With a rate of one image per minute, this microscope is a revolution in the field of 3D electron microscopy. For instance, given a field of view of 40µm width and a pixel size of 5x5x40 nm, the microscope can image 20µm in depth within the sample in an overnight acquisition.
This microscope has been co-funded by the Bettencourt Schueller Society, the GIS IBISA, the Labex INFORM, the PACA area, Aix-Marseille University and the Plan Cancer.
Transmission Electron
Microscope FEI Morgagni 120 kV.
This microscope enables the insertion of samples in less than 20 seconds. It is therefore very useful for grids quality checks. It is equipped with a camera and can be used to acquire images up to a 20 000x magnification.
Sample preparation devices
- Leica EMPact 2, high pressure freezing machine
- Leica AFS 2, freeze substitution apparatus
- Leica AFS, freeze substitution apparatus
- Leica EM AMW, sample preparation automate
- Leica UC7, (cryo)-ultramicrotome
- Leica UCT, (cryo)-ultramicrotome
- Leica UCS, (cryo)-ultramicrotome
- LKB 7801A, knife maker
- Balzers CPD030, critical point dryer
- Edwards S150B, glow discharge and sputter coater apparatus