Platform
Electron microscopy
Scientific manager :
P.F. Lenne
Technical manager :
N. Brouilly
The Electron Microscopy department of the PICsL Imaging facility provides the scientific community with 1. the expertise, 2. the material and 3. electron microscopes necessary for molecular, cellular and tissue-level imaging by electron microscopy.
Our service offer comprises the sample preparation with the following methods: negative staining, plastic embedding, immuno-labelling, high pressure freezing, freeze substitution, (cryo)-ultramictotomy…
We routinely use advanced imaging modes such as electron tomography (ET), cryo-Electron Microscopy (cryo-EM), Scanning Transmission Electron Microscopy (STEM), Scanning Electron Microscopy in « Serial Block-Face » mode (see an example above), Correlative Light and Electron Microscopy (CLEM)…
For the users willing to work autonomously, we offer individual or group trainings for the conventional methods in transmission and scanning electron microscopy.
Please, do not hesitate to contact us: ibdm-plateau-microscopie(at)univ-amu.fr
PUBLICATION
August 22nd, 2018
Delineating the Physiological Roles of the PE and Catalytic Domains of LipY in Lipid Consumption in Mycobacterium-Infected Foamy Macrophages
Santucci P, Diomandé S, Poncin I, Alibaud L, Viljoen A, Kremer L, de Chastellier C, Canaan S.
Infect Immun. 2018 Aug 22;86(9). pii: e00394-18. doi: 10.1128/IAI.00394-18. PMID: 29986895
PUBLICATION
August 19th, 2018
Mitofusin gain and loss of function drive pathogenesis in Drosophila models of CMT2A neuropathy
El Fissi N, Rojo M, Aouane A, Karatas E, Poliacikova G, David C, Royet J, Rival T.
EMBO Rep. 2018 Aug;19(8). pii: e45241. doi: 10.15252/embr.201745241. PMID: 29898954
PUBLICATION
August 1st, 2018
Correct Laminar Positioning in the Neocortex Influences Proper Dendritic and Synaptic Development
Martineau FS, Sahu S, Plantier V, Buhler E, Schaller F, Fournier L, Chazal G, Kawasaki H, Represa A, Watrin F, Manent JB.
Cereb Cortex. 2018 Aug 1;28(8):2976-2990. doi: 10.1093/cercor/bhy113. PMID: 29788228
PUBLICATION
March 27th, 2018
Promoting Myelin Repair through In Vivo Neuroblast Reprogramming.
El Waly B, Cayre M, Durbec P.
Stem Cell Reports. 2018 Mar 27. pii: S2213-6711(18)30106-1. doi: 10.1016/j.stemcr.2018.02.015. PMID: 29606615
PUBLICATION
March 5th, 2018
Nanoplanktonic diatoms are globally overlooked but play a role in spring blooms and carbon export
Leblanc K, Quéguiner B, Diaz F, Cornet V, Michel-Rodriguez M, Durrieu de Madron X, Bowler C, Malviya S, Thyssen M, Grégori G, Rembauville M, Grosso O, Poulain J, de Vargas C, Pujo-Pay M, Conan P.
Nat Commun. 2018 Mar 5;9(1):953. doi: 10.1038/s41467-018-03376-9. PMID: 29507291
PUBLICATION
February 15th, 2018
Tailoring drug release rates in hydrogel-based therapeutic delivery applications using graphene oxide
Puvirajesinghe TM, Zhi ZL, Craster RV, Guenneau S.
J R Soc Interface. 2018 Feb;15(139). pii: 20170949. doi: 10.1098/rsif.2017.0949. PMID: 29445040
PUBLICATION
June 19th, 2017
Characterization and photodegradation mechanism of three Algerian wood species
Yasmina Ouadou, Djamel Aliouche, Marie-France Thevenon, Mohamed Djillali
Journal of Wood Science
PUBLICATION
March 30th, 2017
Epithelial tension in the second heart field promotes mouse heart tube elongation.
Francou A, De Bono C, Kelly RG.
Nat Commun. 2017 Mar 30;8:14770. PMID: 28357999
PUBLICATION
November 6th, 2016
The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages
Roux AL, Viljoen A, Bah A, Simeone R, Bernut A, Laencina L, Deramaudt T, Rottman M, Gaillard JL, Majlessi L, Brosch R, Girard-Misguich F, Vergne I, de Chastellier C, Kremer L, Herrmann JL.
Open Biol. 2016 Nov;6(11). pii: 160185. PMID: 27906132
PUBLICATION
November 5th, 2016
MAB_3551c encodes the primary triacylglycerol synthase involved in lipid accumulation in Mycobacterium abscessus
Viljoen A, Blaise M, de Chastellier C, Kremer L.
Mol Microbiol. 2016 Nov;102(4):611-627. doi: 10.1111/mmi.13482. PMID: 27513974
PUBLICATION
November 1st, 2016
Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness.
Leca J, Martinez S, Lac S, Nigri J, Secq V, Rubis M, Bressy C, Sergé A, Lavaut MN, Dusetti N, Loncle C, Roques J, Pietrasz D, Bousquet C, Garcia S, Granjeaud S, Ouaissi M, Bachet JB, Brun C, Iovanna JL, Zimmermann P, Vasseur S, Tomasini R.
J Clin Invest. 2016 Nov 1;126(11):4140-4156. doi: 10.1172/JCI87734. Epub 2016 Oct 4. PMID: 27701147
PUBLICATION
October 7th, 2016
Experimental Models of Foamy Macrophages and Approaches for Dissecting the Mechanisms of Lipid Accumulation and Consumption during Dormancy and Reactivation of Tuberculosis
Santucci P, Bouzid F, Smichi N, Poncin I, Kremer L, De Chastellier C, Drancourt M, Canaan S.
Front Cell Infect Microbiol. 2016 Oct 7;6:122. eCollection 2016. PMID: 27774438
PUBLICATION
September 2nd, 2016
Biochemical and Spectroscopic Characterization of Highly Stable Photosystem II Supercomplexes from Arabidopsis
Crepin A, Santabarbara S, Caffarri S.
J Biol Chem. 2016 Sep 2;291(36):19157-71. doi: 10.1074/jbc.M116.738054. PMID: 27432883
PUBLICATION
June 7th, 2016
LAMP5 Fine-Tunes GABAergic Synaptic Transmission in Defined Circuits of the Mouse Brain.
Tiveron MC, Beurrier C, Céni C, Andriambao N, Combes A, Koehl M, Maurice N, Gatti E, Abrous DN, Kerkerian-Le Goff L, Pierre P, Cremer H.
PLoS One. 2016 Jun 7;11(6):e0157052. PMID: 27272053
PUBLICATION
May 6th, 2016
Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution
Baati T, Al-Kattan A, Esteve MA, Njim L, Ryabchikov Y, Chaspoul F, Hammami M, Sentis M, Kabashin AV, Braguer D.
Sci Rep. 2016 May 6;6:25400. doi: 10.1038/srep25400. PMID: 27151839
PUBLICATION
March 5th, 2016
Insights into the smooth-to-rough transitioning in Mycobacterium bolletii unravels a functional Tyr residue conserved in all mycobacterial MmpL family members
Bernut A, Viljoen A, Dupont C, Sapriel G, Blaise M, Bouchier C, Brosch R, de Chastellier C, Herrmann JL, Kremer L.
Mol Microbiol. 2016 Mar;99(5):866-83. doi: 10.1111/mmi.13283. PMID: 26585558
PUBLICATION
November 25th, 2015
Brucella discriminates between mouse dendritic cell subsets upon in vitro infection
Papadopoulos A, Gagnaire A, Degos C, de Chastellier C, Gorvel JP.
Virulence. 2016;7(1):33-44. doi: 10.1080/21505594.2015.1108516. PMID: 26606688
PUBLICATION
October 10th, 2015
Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis
Lemmer Y, Kalombo L, Pietersen RD, Jones AT, Semete-Makokotlela B, Van Wyngaardt S, Ramalapa B, Stoltz AC, Baker B, Verschoor JA5, Swai HS, de Chastellier C.
J Control Release. 2015 Aug 10;211:94-104. doi: 10.1016/j.jconrel.2015.06.005. PMID: 26055640
PUBLICATION
June 7th, 2015
Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1
Seillier M, Pouyet L, N'Guessan P, Nollet M, Capo F, Guillaumond F, Peyta L, Dumas JF, Varrault A, Bertrand G, Bonnafous S, Tran A, Meur G, Marchetti P, Ravier MA, Dalle S, Gual P, Muller D, Rutter GA, Servais S, Iovanna JL, Carrier A.
EMBO Mol Med. 2015 Jun;7(6):802-18. doi: 10.15252/emmm.201404318. PMID: 25828351
PUBLICATION
January 22nd, 2015
PINK1-induced mitophagy promotes neuroprotection in Huntington's disease.
Khalil B, El Fissi N, Aouane A, Cabirol-Pol MJ, Rival T, Liévens JC.
Cell Death Dis. 2015 Jan 22;6:e1617. doi: 10.1038/cddis.2014.581. PMID: 25611391
PUBLICATION
December 9th, 2014
Diversity of Magnetotactic Bacteria from a French Pristine Mediterranean Area.
Fuduche M, Postec A, Davidson S, Chauvin JP, Galès G, Hirschler-Réa A, Ollivier B, Wu LF, Pradel N.
Curr Microbiol. 2014 Dec 9. [Epub ahead of print] PMID: 25487117
PUBLICATION
December 1st, 2014
Loss of Krox20 results in aortic valve regurgitation and impaired transcriptional activation of fibrillar collagen genes.
Odelin G, Faure E, Kober F, Maurel-Zaffran C, Théron A, Coulpier F, Guillet B, Bernard M, Avierinos JF, Charnay P, Topilko P, Zaffran S.
Cardiovasc Res. 2014 Dec 1;104(3):443-55. PMID: 25344368
PUBLICATION
November 1st, 2014
TBX1 regulates epithelial polarity and dynamic basal filopodia in the second heart field.
Francou A, Saint-Michel E, Mesbah K, Kelly RG.
Development. 2014 Nov;141(22):4320-31. doi: 10.1242/dev.115022. PMID: 25371366
PUBLICATION
August 1st, 2014
Repair of the injured spinal cord by implantation of a synthetic degradable block copolymer in rat.
Pertici V, Trimaille T, Laurin J, Felix MS, Marqueste T, Pettmann B, Chauvin JP, Gigmes D, Decherchi P.
Biomaterials. 2014 Aug;35(24):6248-58. PMID: 24814425
PUBLICATION
July 29th, 2014
A dual role for planar cell polarity genes in ciliated cells
Boutin C, Labedan P, Dimidschstein J, Richard F, Cremer H, André P, Yang Y, Montcouquiol M, Goffinet AM, Tissir F.
Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):E3129-38. doi: 10.1073/pnas.1404988111. PMID: 25024228
PUBLICATION
July 15th, 2014
CRB2 acts as a modifying factor of CRB1-related retinal dystrophies in mice.
Pellissier LP, Lundvig DM, Tanimoto N, Klooster J, Vos RM, Richard F, Sothilingam V, Garcia Garrido M, Le Bivic A, Seeliger MW, Wijnholds J.
Hum Mol Genet. 2014 Jul 15;23(14):3759-71. PMID: 24565864
PUBLICATION
March 18th, 2014
Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology.
Legendre M, Bartoli J, Shmakova L, Jeudy S, Labadie K, Adrait A, Lescot M, Poirot O, Bertaux L, Bruley C, Couté Y, Rivkina E, Abergel C, Claverie JM.
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4274-9. PMID: 24591590
PUBLICATION
March 18th, 2014
Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2.
Ghossoub R, Lembo F, Rubio A, Gaillard CB, Bouchet J, Vitale N, Slavík J, Machala M, Zimmermann P.
Nat Commun. 2014 Mar 18;5:3477. PMID: 24637612
PUBLICATION
February 6th, 2014
Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context.
Castillo-Briceno P, Kodjabachian L.
Front Zool. 2014 Feb 6;11(1):9. PMID: 24502321
PUBLICATION
February 1st, 2014
Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection
Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O.
PLoS Pathog. 2014 Feb 20;10(2):e1003928. PMID: 24586151
PUBLICATION
February 1st, 2014
Reversible lipid accumulation and associated division arrest of Mycobacterium avium in lipoprotein-induced foamy macrophages may resemble key events during latency and reactivation of tuberculosis.
Caire-Brändli I, Papadopoulos A, Malaga W, Marais D, Canaan S, Thilo L, de Chastellier C.
Infect Immun. 2014 Feb;82(2):476-90. PMID: 24478064
PUBLICATION
January 12th, 2014
Drebrin E depletion in human intestinal epithelial cells mimics Rab8a loss of function.
Vacca B, Bazellières E, Nouar R, Harada A, Massey-Harroche D, Le Bivic A.
Hum Mol Genet. 2014 Jan 12. PMID: 24399445
PUBLICATION
November 14th, 2013
Regulation of mycolactone, the Mycobacterium ulcerans toxin, depends on nutrient source.
Deshayes C, Angala SK, Marion E, Brandli I, Babonneau J, Preisser L, Eyangoh S, Delneste Y, Legras P, De Chastellier C, Stinear TP, Jackson M, Marsollier L.
PLoS Negl Trop Dis. 2013 Nov 14;7(11):e2502. PMID: 24244764
PUBLICATION
September 27th, 2013
Pandoraviruses: amoeba viruses with genomes up to 2.5 Mb reaching that of parasitic eukaryotes.
Philippe N, Legendre M, Doutre G, Couté Y, Poirot O, Lescot M, Arslan D, Seltzer V, Bertaux L, Bruley C, Garin J, Claverie JM, Abergel C.
Science. 2013 Jul 19;341(6143):281-6. PMID: 23869018
PUBLICATION
June 25th, 2013
Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes.
Santini S, Jeudy S, Bartoli J, Poirot O, Lescot M, Abergel C, Barbe V, Wommack KE, Noordeloos AA, Brussaard CP, Claverie JM.
Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10800-5. PMID: 23754393
PUBLICATION
April 1st, 2013
Pathogenic brucellae replicate in human trophoblasts.
Salcedo SP, Chevrier N, Lacerda TL, Ben Amara A, Gerart S, Gorvel VA, de Chastellier C, Blasco JM, Mege JL, Gorvel JP.
J Infect Dis. 2013 Apr;207(7):1075-83. PMID: 23303808
PUBLICATION
January 1st, 2013
Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene.
Alves CH, Sanz AS, Park B, Pellissier LP, Tanimoto N, Beck SC, Huber G, Murtaza M, Richard F, Sridevi Gurubaran I, Garcia Garrido M, Levelt CN, Rashbass P, Le Bivic A, Seeliger MW, Wijnholds J.
Hum Mol Genet. 2013 Jan 1;22(1):35-50. PMID: 23001562
PUBLICATION
December 19th, 2012
Structure of the dimeric RC-LH1-PufX complex from Rhodobaca bogoriensis investigated by electron microscopy.
Semchonok DA, Chauvin JP, Frese RN, Jungas C, Boekema EJ.
Philos Trans R Soc Lond B Biol Sci. 2012 Dec 19 PMID: 23148268
PUBLICATION
July 27th, 2012
Phosphorylation of mycobacterial PcaA inhibits mycolic acid cyclopropanation: consequences for intracellular survival and for phagosome maturation block.
Corrales RM, Molle V, Leiba J, Mourey L, de Chastellier C, Kremer L.
J Biol Chem. 2012 Jul 27;287(31):26187-99. PMID: 22621931
PUBLICATION
March 20th, 2012
Metallobiology of host-pathogen interactions: an intoxicating new insight.
Botella H, Stadthagen G, Lugo-Villarino G, de Chastellier C, Neyrolles O.
Trends Microbiol. 2012 Mar;20(3):106-12. PMID: 22305804
PUBLICATION
February 1st, 2012
Olesoxime accelerates myelination and promotes repair in models of demyelination.
Magalon K, Zimmer C, Cayre M, Khaldi J, Bourbon C, Robles I, Tardif G, Viola A, Pruss RM, Bordet T, Durbec P.
Ann Neurol. 2012 Feb;71(2):213-26. PMID: 22367994
PUBLICATION
January 28th, 2012
[A novel role for transition metals in anti-microbial immunity].
Botella H, Stadthagen G, de Chastellier C, Neyrolles O.
Med Sci (Paris). 2012 Jan;28(1):18-21. PMID: 22289820
PUBLICATION
January 1st, 2012
Hippocampal GABAergic neurons are susceptible to amyloid-β toxicity in vitro and are decreased in number in the Alzheimer's disease TgCRND8 mouse model.
Krantic S, Isorce N, Mechawar N, Davoli MA, Vignault E, Albuquerque M, Chabot JG, Moyse E, Chauvin JP, Aubert I, McLaurin J, Quirion R.
J Alzheimers Dis. 2012;29(2):293-308. PMID: 22232004
PUBLICATION
September 15th, 2011
Mycobacterial p(1)-type ATPases mediate resistance to zinc poisoning in human macrophages.
Botella H, Peyron P, Levillain F, Poincloux R, Poquet Y, Brandli I, Wang C, Tailleux L, Tilleul S, Charrière GM, Waddell SJ, Foti M, Lugo-Villarino G, Gao Q, Maridonneau-Parini I, Butcher PD, Castagnoli PR, Gicquel B, de Chastellier C, Neyrolles O.
Cell Host Microbe. 2011 Sep 15;10(3):248-59. PMID: 21925112
PUBLICATION
June 5th, 2011
Body plan innovation in treehoppers through the evolution of an extra wing-like appendage.
Prud'homme B, Minervino C, Hocine M, Cande JD, Aouane A, Dufour HD, Kassner VA, Gompel N.
Nature. 2011 May 5;473(7345):83-6. PMID: 21544145
PUBLICATION
May 1st, 2011
Trafficking and secretion of matrix metalloproteinase-2 in olfactory ensheathing glial cells: A role in cell migration?
Gueye Y, Ferhat L, Sbai O, Bianco J, Ould-Yahoui A, Bernard A, Charrat E, Chauvin JP, Risso JJ, Féron F, Rivera S, Khrestchatisky M.
Glia. 2011 May;59(5):750-70. PMID: 21360755
PUBLICATION
April 15th, 2011
Neuronal chloride accumulation and excitatory GABA underlie aggravation of neonatal epileptiform activities by phenobarbital
Nardou R, Yamamoto S, Chazal G, Bhar A, Ferrand N, Dulac O, Ben-Ari Y, Khalilov I.
Brain. 2011 Apr;134(Pt 4):987-1002. doi: 10.1093/brain/awr041. PMID: 21436113
PUBLICATION
April 12th, 2011
Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice.
Ganay T, Boizot A, Burrer R, Chauvin JP, Bomont P.
Mol Neurodegener. 2011 Apr 12;6:25. PMID: 21486449
PUBLICATION
March 1st, 2011
Inner-membrane proteins PMI/TMEM11 regulate mitochondrial morphogenesis independently of the DRP1/MFN fission/fusion pathways.
Rival T, Macchi M, Arnauné-Pelloquin L, Poidevin M, Maillet F, Richard F, Fatmi A, Belenguer P, Royet J.
EMBO Rep. 2011 Mar;12(3):223-30. PMID: 21274005
PUBLICATION
October 1st, 2010
Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.
Nisoli I, Chauvin JP, Napoletano F, Calamita P, Zanin V, Fanto M, Charroux B.
Cell Death Differ. 2010 Oct;17(10):1577-87. PMID: 20339376
PUBLICATION
July 15th, 2010
The virulence protein SopD2 regulates membrane dynamics of Salmonella-containing vacuoles.
Schroeder N, Henry T, de Chastellier C, Zhao W, Guilhon AA, Gorvel JP, Méresse S.
PLoS Pathog. 2010 Jul 15;6(7):e1001002 PMID: 20664790
PUBLICATION
July 15th, 2010
Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein.
Sedjaï F, Acquaviva C, Chevrier V, Chauvin JP, Coppin E, Aouane A, Coulier F, Tolun A, Pierres M, Birnbaum D, Rosnet O.
J Cell Sci. 2010 Jul 15;123(Pt 14):2391-401. PMID: 20551181
PUBLICATION
April 7th, 2010
A polycystin-2 (TRPP2) dimerization domain essential for the function of heteromeric polycystin complexes.
Giamarchi A, Feng S, Rodat-Despoix L, Xu Y, Bubenshchikova E, Newby LJ, Hao J, Gaudioso C, Crest M, Lupas AN, Honoré E, Williamson MP, Obara T, Ong AC, Delmas P.
EMBO J. 2010 Apr 7;29(7):1176-91. doi: 10.1038/emboj.2010.18. PMID: 20168298
PUBLICATION
February 1st, 2010
Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.
Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M.
Glia. 2010 Feb;58(3):344-66. PMID: 19780201
PUBLICATION
August 11th, 2009
GABA action in immature neocortical neurons directly depends on the availability of ketone bodies.
Rheims S, Holmgren CD, Chazal G, Mulder J, Harkany T, Zilberter T, Zilberter Y.
J Neurochem. 2009 Aug;110(4):1330-8. PMID: 19558450
PUBLICATION
June 5th, 2009
The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication.
Fugier E, Salcedo SP, de Chastellier C, Pophillat M, Muller A, Arce-Gorvel V, Fourquet P, Gorvel JP.
PLoS Pathog. 2009 Jun;5(6):e1000487. PMID: 19557163
PUBLICATION
June 1st, 2009
PSD95beta regulates plasma membrane Ca2+ pump localization at the photoreceptor synapse.
Aartsen WM, Arsanto JP, Chauvin JP, Vos RM, Versteeg I, Cardozo BN, Bivic AL, Wijnholds J.
Mol Cell Neurosci. 2009 Jun;41(2):156-65. PMID: 19233278
PUBLICATION
April 15th, 2009
The centrosomal FOP protein is required for cell cycle progression and survival.
Acquaviva C, Chevrier V, Chauvin JP, Fournier G, Birnbaum D, Rosnet O.
Cell Cycle. 2009 Apr 15;8(8):1217-27. PMID: 19305129
PUBLICATION
April 1st, 2009
Ajuba: a new microtubule-associated protein that interacts with BUBR1 and Aurora B at kinetochores in metaphase.
Ferrand A, Chevrier V, Chauvin JP, Birnbaum D.
Biol Cell. 2009 Apr;101(4):221-35. PMID: 18710370
PUBLICATION
December 1st, 2008
Vesicular trafficking and secretion of matrix metalloproteinases-2, -9 and tissue inhibitor of metalloproteinases-1 in neuronal cells.
Sbai O, Ferhat L, Bernard A, Gueye Y, Ould-Yahoui A, Thiolloy S, Charrat E, Charton G, Tremblay E, Risso JJ, Chauvin JP, Arsanto JP, Rivera S, Khrestchatisky M.
Mol Cell Neurosci. 2008 Dec;39(4):549-68. PMID: 18817873
PUBLICATION
November 4th, 2008
Foamy macrophages from tuberculous patients' granulomas constitute a nutrient-rich reservoir for M. tuberculosis persistence.
Peyron P, Vaubourgeix J, Poquet Y, Levillain F, Botanch C, Bardou F, Daffé M, Emile JF, Marchou B, Cardona PJ, de Chastellier C, Altare F.
PLoS Pathog. 2008 Nov;4(11):e1000204. PMID: 19002241
PUBLICATION
October 1st, 2008
Marked mitochondrial alterations upon starvation without cell death, caspases or Bcl-2 family members.
Kosta A, Luciani MF, Geerts WJ, Golstein P.
Biochim Biophys Acta. 2008 Oct;1783(10):2013-9. PMID: 18652851
PUBLICATION
January 1st, 2008
Functional role of the PE domain and immunogenicity of the Mycobacterium tuberculosis triacylglycerol hydrolase LipY.
Mishra KC, de Chastellier C, Narayana Y, Bifani P, Brown AK, Besra GS, Katoch VM, Joshi B, Balaji KN, Kremer L.
Infect Immun. 2008 Jan;76(1):127-40. PMID: 17938218
PUBLICATION
January 1st, 2008
Analysis of autophagic and necrotic cell death in Dictyostelium.
Giusti C, Kosta A, Lam D, Tresse E, Luciani MF, Golstein P.
Methods Enzymol. 2008;446:1-15. PMID: 18603113
PUBLICATION
January 1st, 2008
Autophagy and autophagic cell death in Dictyostelium.
Tresse E, Giusti C, Kosta A, Luciani MF, Golstein P.
Methods Enzymol. 2008;451:343-58. PMID: 19185731
PUBLICATION
April 17th, 2007
From autophagic to necrotic cell death in Dictyostelium.
Tresse E, Kosta A, Luciani MF, Golstein P.
Semin Cancer Biol. 2007 Apr;17(2):94-100. PMID: 17150370
PUBLICATION
April 9th, 2007
Birc1e/Naip5 rapidly antagonizes modulation of phagosome maturation by Legionella pneumophila.
Fortier A, de Chastellier C, Balor S, Gros P.
Cell Microbiol. 2007 Apr;9(4):910-23. PMID: 17087731
PUBLICATION
March 15th, 2007
Nkx2.5 cell-autonomous gene function is required for the postnatal formation of the peripheral ventricular conduction system.
Meysen S, Marger L, Hewett KW, Jarry-Guichard T, Agarkova I, Chauvin JP, Perriard JC, Izumo S, Gourdie RG, Mangoni ME, Nargeot J, Gros D, Miquerol L.
Dev Biol. 2007 Mar 15;303(2):740-53. PMID: 17250822
PUBLICATION
January 1st, 2007
Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo.
Miranda-Tello E, Fardeau ML, Joulian C, Magot M, Thomas P, Tholozan JL, Ollivier B.
Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):40-4. PMID: 17220438
PUBLICATION
March 15th, 2006
Atrophin contributes to the negative regulation of epidermal growth factor receptor signaling in Drosophila.
Charroux B, Freeman M, Kerridge S, Baonza A.
Dev Biol. 2006 Mar 15;291(2):278-90. PMID: 16445904
PUBLICATION
February 8th, 2006
Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts.
Lapaque N, Forquet F, de Chastellier C, Mishal Z, Jolly G, Moreno E, Moriyon I, Heuser JE, He HT, Gorvel JP.
Cell Microbiol. 2006 Feb;8(2):197-206. PMID: 16441431
PUBLICATION
February 8th, 2006
Cholesterol depletion in Mycobacterium avium-infected macrophages overcomes the block in phagosome maturation and leads to the reversible sequestration of viable mycobacteria in phagolysosome-derived autophagic vacuoles.
de Chastellier C, Thilo L.
Cell Microbiol. 2006 Feb;8(2):242-56. PMID: 16441435
PUBLICATION
February 1st, 2006
Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation.
Pilot F, Philippe JM, Lemmers C, Chauvin JP, Lecuit T.
Development. 2006 Feb;133(4):711-23. PMID: 16421189
PUBLICATION
October 7th, 2005
Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.
Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S.
Cell. 2005 Oct 7;123(1):157-70. PMID: 16213220
PUBLICATION
August 1st, 2005
Identification and structural characterization of an unusual mycobacterial monomeromycolyl-diacylglycerol.
Kremer L, de Chastellier C, Dobson G, Gibson KJ, Bifani P, Balor S, Gorvel JP, Locht C, Minnikin DE, Besra GS.
Mol Microbiol. 2005 Aug;57(4):1113-26. PMID: 16091048
PUBLICATION
March 28th, 2005
mCD24 regulates proliferation of neuronal committed precursors in the subventricular zone.
Nieoullon V, Belvindrah R, Rougon G, Chazal G.
Mol Cell Neurosci. 2005 Mar;28(3):462-74. PMID: 15737737
PUBLICATION
March 1st, 2005
Expanded polyglutamine peptides disrupt EGF receptor signaling and glutamate transporter expression in Drosophila.
Liévens JC1, Rival T, Iché M, Chneiweiss H, Birman S.
Hum Mol Genet. 2005 Mar 1;14(5):713-24. PMID: 15677486
PUBLICATION
January 1st, 2005
Bacteria spurned by self-absorbed cells.
Gorvel JP, de Chastellier C.
Nat Med. 2005 Jan;11(1):18-9. PMID: 15635436
PUBLICATION
November 12th, 2004
Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.
Kosta A, Roisin-Bouffay C, Luciani MF, Otto GP, Kessin RH, Golstein P.
J Biol Chem. 2004 Nov 12;279(46):48404-9. PMID: 15358773
PUBLICATION
May 1st, 2004
Mycobacterium tuberculosis and Mycobacterium avium modify the composition of the phagosomal membrane in infected macrophages by selective depletion of cell surface-derived glycoconjugates.
Pietersen R, Thilo L, de Chastellier C.
Eur J Cell Biol. 2004 May;83(4):153-8. PMID: 15260437
PUBLICATION
January 1st, 2004
Petrotoga mexicana sp. nov., a novel thermophilic, anaerobic and xylanolytic bacterium isolated from an oil-producing well in the Gulf of Mexico.
Miranda-Tello E, Fardeau ML, Thomas P, Ramirez F, Casalot L, Cayol JL, Garcia JL, Ollivier B.
Int J Syst Evol Microbiol. 2004 Jan;54(Pt 1):169-74. PMID: 14742476
PUBLICATION
October 28th, 2003
Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis.
Pelissier A, Chauvin JP, Lecuit T.
Curr Biol. 2003 Oct 28;13(21):1848-57. PMID: 14588240
PUBLICATION
August 18th, 2003
Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum.
Celli J, de Chastellier C, Franchini DM, Pizarro-Cerda J, Moreno E, Gorvel JP.
J Exp Med. 2003 Aug 18;198(4):545-56. PMID: 12925673
PUBLICATION
July 1st, 2003
Production of a polyunsaturated isoprenoid wax ester during aerobic metabolism of squalene by Marinobacter squalenivorans sp. nov.
Rontani JF, Mouzdahir A, Michotey V, Caumette P, Bonin P.
Appl Environ Microbiol. 2003 Jul;69(7):4167-76. PMID: 12839795
PUBLICATION
July 1st, 2003
The type 3 inositol 1,4,5-trisphosphate receptor is concentrated at the tight junction level in polarized MDCK cells.
Colosetti P, Tunwell RE, Cruttwell C, Arsanto JP, Mauger JP, Cassio D.
J Cell Sci. 2003 Jul 1;116(Pt 13):2791-803. PMID: 12759372
PUBLICATION
March 31st, 2003
Dictyostelium cell death: early emergence and demise of highly polarized paddle cells.
Levraud JP, Adam M, Luciani MF, de Chastellier C, Blanton RL, Golstein P.
J Cell Biol. 2003 Mar 31;160(7):1105-14. PMID: 12654899
PUBLICATION
August 15th, 2002
Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.
Soustelle L, Besson MT, Rival T, Birman S.
Dev Biol. 2002 Aug 15;248(2):294-306. PMID: 12167405
PUBLICATION
July 1st, 2002
Pericardin, a Drosophila type IV collagen-like protein is involved in the morphogenesis and maintenance of the heart epithelium during dorsal ectoderm closure.
Chartier A, Zaffran S, Astier M, Sémériva M, Gratecos D.
Development. 2002 Jul;129(13):3241-53. PMID: 12070098
PUBLICATION
May 30th, 2002
Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure.
Morozov Y, Khalilov I, Ben-Ari Y, Represa A.
J Neurosci Methods. 2002 May 30;117(1):81-5. PMID: 12084567
PUBLICATION
May 1st, 2002
Increased neurogenesis in adult mCD24-deficient mice.
Belvindrah R, Rougon G, Chazal G.
J Neurosci. 2002 May 1;22(9):3594-607. PMID: 11978835
PUBLICATION
April 15th, 2002
TCR signal initiation machinery is pre-assembled and activated in a subset of membrane rafts.
Drevot P, Langlet C, Guo XJ, Bernard AM, Colard O, Chauvin JP, Lasserre R, He HT.
EMBO J. 2002 Apr 15;21(8):1899-908. PMID: 11953309
PUBLICATION
March 1st, 2002
PrP expression and replication by Schwann cells: implications in prion spreading.
Follet J, Lemaire-Vieille C, Blanquet-Grossard F, Podevin-Dimster V, Lehmann S, Chauvin JP, Decavel JP, Varea R, Grassi J, Fontès M, Cesbron JY.
J Virol. 2002 Mar;76(5):2434-9. PMID: 11836421
Transmission Electron
Microscope FEI Tecnai G2 200 kV.
This microscope is designed for advanced imaging modes such as electron tomography, STEM and cryo-electron microscopy. With its 200kV gun and the optical quality of its column, it can reach a magnification of about 200 000x. It is equipped with an Olympus Veleta 2k camera and an FEI Eagle 4k camera.
This microscope has been co-funded by the Plan Cancer, the INSB, the FEDER and the IBDM.
Scanning Electron
Microscope FEI Teneo VS.
With this microscope, our users can aqcuire conventional MEB images of an outstanding quality. Moreover, this microscope is equipped with an automatized ultra-microtomy module. By the technique of Serial Block-Face, the microscope autonomously alternate between cutting and imaging steps of an embedded sample. With a rate of one image per minute, this microscope is a revolution in the field of 3D electron microscopy. For instance, given a field of view of 40µm width and a pixel size of 5x5x40 nm, the microscope can image 20µm in depth within the sample in an overnight acquisition.
This microscope has been co-funded by the Bettencourt Schueller Society, the GIS IBISA, the Labex INFORM, the PACA area, Aix-Marseille University and the Plan Cancer.
Transmission Electron
Microscope FEI Morgagni 120 kV.
This microscope enables the insertion of samples in less than 20 seconds. It is therefore very useful for grids quality checks. It is equipped with a camera and can be used to acquire images up to a 20 000x magnification.
Sample preparation devices
- Leica EMPact 2, high pressure freezing machine
- Leica AFS 2, freeze substitution apparatus
- Leica AFS, freeze substitution apparatus
- Leica EM AMW, sample preparation automate
- Leica UC7, (cryo)-ultramicrotome
- Leica UCT, (cryo)-ultramicrotome
- Leica UCS, (cryo)-ultramicrotome
- LKB 7801A, knife maker
- Balzers CPD030, critical point dryer
- Edwards S150B, glow discharge and sputter coater apparatus
