Platform

Xenopus facility

Scientific manager : L. Kodjabachian
Technical manager : F. Roguet

xenope-1The Xenopus facility is located at the underground level of the large TPR2 building. It is equipped with two independent sets of tanks (9 and 15 tanks). Each tank has a capacity of 100 litres and can host 10 to 12 females or 20 to 25 males, which allows us to keep a total of 250 to 300 Xenopus laevis. Each tank is equipped with a dripping water supply, which allows complete water replacement within a day.

The water going through the system is first allowed to sit in a very large tank to favour chlorine depletion. Animals are fed with granulates for fish. Neon light is supplied on a 12h/12h dark/light cycle. Temperature in the room is maintained at 20°C throughout the year.

OLYMPUS DIGITAL CAMERAFrançois Graziani is in charge of animal care and cleaning of the facility, under the supervision of Laurent Kodjabachian,who is holding the legal permits to run the facility.

In Kodjabachian’s team, we use the Xenopus embryo as a model for early vertebrate development. Twice a week on average, in vitro fertilisations are performed, which generates thousands of embryos for micro-injections and embryological experiments. Following egg laying, females are allowed to rest for at least three months, to replete ovocytes stock. We currently not involved in breeding, instead we keep adult animals. All our individuals are wild-type, except a for few albinos.

Members more

Patrick Gariglio Florian Roguet
Laurent Kodjabachian
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Laurent Kodjabachian

Researcher

Patrick Gariglio
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Patrick Gariglio

Technical staff

Florian Roguet
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Florian Roguet

Technical staff